OBJECTIVE: This study was designed to evaluate the toxic effects of Atrazine (ATZ) on the reproductive system of male rats. METHODS: Male Sprague-Dawley rats were exposed to ATZ by gavage at dosages of 0, 38.5, 77, and 154 mg/kg bw/day for 30 d. The toxic effects of ATZ to rats were assessed through histopathologcal observation, spermatozoa quality evaluation, testicular marker enzyme indicators, antioxidant capacity and reproductive hormone levels. RESULTS: Significant adverse effects on reproductive system were observed in rats exposed to ATZ at different dosages compared with 0 mg/kg group, including an irregular and disordered arrangement of the seminiferous epithelium in 154 mg/kg group; a decreased spermatozoa number and an increased spermatozoa abnormality rate in 77 and 154 mg/kg groups; decreased levels of acid phosphatase (ACP), alkaline phosphatase (AKP), lactic dehydrogenase (LDH), and succinate dehydrogenase (SDH) with the increasing of ATZ concentration; a decreased level of total antioxidant capacity (TAC) in a dose-dependent manner, and a decreased reduced glutathione (GSH) level and an increased malondialdehyde (MDA) content in 154 mg/kg group; and decreased serum levels of testosterone (T) and inhibin-B (INH-B) and an increased serum level of follicle stimulating hormone (FSH) in 77 and 154 mg/kg groups, and an increased serum level of luteinizing hormone (LH) in 154 mg/kg group. CONCLUSION: These results suggested that relatively high doses of ATZ could exert reproductive toxicity of male rats.
OBJECTIVE: This study was designed to evaluate the toxic effects of Atrazine (ATZ) on the reproductive system of male rats. METHODS: Male Sprague-Dawley rats were exposed to ATZ by gavage at dosages of 0, 38.5, 77, and 154 mg/kg bw/day for 30 d. The toxic effects of ATZ to rats were assessed through histopathologcal observation, spermatozoa quality evaluation, testicular marker enzyme indicators, antioxidant capacity and reproductive hormone levels. RESULTS: Significant adverse effects on reproductive system were observed in rats exposed to ATZ at different dosages compared with 0 mg/kg group, including an irregular and disordered arrangement of the seminiferous epithelium in 154 mg/kg group; a decreased spermatozoa number and an increased spermatozoa abnormality rate in 77 and 154 mg/kg groups; decreased levels of acid phosphatase (ACP), alkaline phosphatase (AKP), lactic dehydrogenase (LDH), and succinate dehydrogenase (SDH) with the increasing of ATZ concentration; a decreased level of total antioxidant capacity (TAC) in a dose-dependent manner, and a decreased reduced glutathione (GSH) level and an increased malondialdehyde (MDA) content in 154 mg/kg group; and decreased serum levels of testosterone (T) and inhibin-B (INH-B) and an increased serum level of follicle stimulating hormone (FSH) in 77 and 154 mg/kg groups, and an increased serum level of luteinizing hormone (LH) in 154 mg/kg group. CONCLUSION: These results suggested that relatively high doses of ATZ could exert reproductive toxicity of male rats.
Authors: Katharine A Horzmann; Leeah S Reidenbach; Devang H Thanki; Anna E Winchester; Brad A Qualizza; Geoffrey A Ryan; Kaitlyn E Egan; Victoria E Hedrick; Tiago J P Sobreira; Samuel M Peterson; Gregory J Weber; Sara E Wirbisky-Hershberger; Maria S Sepúlveda; Jennifer L Freeman Journal: J Proteomics Date: 2018-07-20 Impact factor: 4.044
Authors: Margaux McBirney; Stephanie E King; Michelle Pappalardo; Elizabeth Houser; Margaret Unkefer; Eric Nilsson; Ingrid Sadler-Riggleman; Daniel Beck; Paul Winchester; Michael K Skinner Journal: PLoS One Date: 2017-09-20 Impact factor: 3.240
Authors: Katharine A Horzmann; Li F Lin; Boghos Taslakjian; Chongli Yuan; Jennifer L Freeman Journal: Cell Biol Toxicol Date: 2020-07-31 Impact factor: 6.819