Literature DB >> 24757296

Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody.

Maryam Farahnak Zarabi1, Ali Farhangi2, Samaneh Khademi Mazdeh2, Zahra Ansarian3, Davod Zare2, Mohammad Reza Mehrabi2, Azim Akbarzadeh2.   

Abstract

Carboxylate-modified gold nanoparticles (GNPs) were synthesized in a simple one-step process based on the reduction of tetrachloroauric acid by aspartic acid in water. GNPs were identified by UV-Vis spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy. Conjugation of protein molecules with functionalized nanoparticles was performed through electrostatic interaction. The GNP-protein conjugates were characterized by gel electrophoresis. The interaction between functionalized GNPs and protein molecules lead to conformational transition of protein structure after conjugation of protein with GNPs. This process was investigated by fluorescence spectroscopy and circular dichroism spectroscopy.

Entities:  

Keywords:  Aspartic acid; Bioconjugation; Colloid; FVIII protein; Gold nanoparticles

Year:  2013        PMID： 24757296      PMCID： PMC3990797          DOI： 10.1007/s12291-013-0323-2

Source DB:  PubMed          Journal:  Indian J Clin Biochem        ISSN： 0970-1915


  16 in total

1.  "Plugging into Enzymes": nanowiring of redox enzymes by a gold nanoparticle.

Authors:  Yi Xiao; Fernando Patolsky; Eugenii Katz; James F Hainfeld; Itamar Willner
Journal:  Science       Date:  2003-03-21       Impact factor: 47.728

2.  Labeling ribonuclease S with a 3 nm Au nanoparticle by two-step assembly.

Authors:  Marie-Eve Aubin; Diana G Morales; Kimberly Hamad-Schifferli
Journal:  Nano Lett       Date:  2005-03       Impact factor: 11.189

3.  Rigid, specific, and discrete gold nanoparticle/antibody conjugates.

Authors:  Christopher J Ackerson; Pablo D Jadzinsky; Grant J Jensen; Roger D Kornberg
Journal:  J Am Chem Soc       Date:  2006-03-01       Impact factor: 15.419

4.  Gold nanoparticle-based surface enhanced Raman scattering spectroscopic assay for the detection of protein-protein interactions.

Authors:  Tao Li; Liping Guo; Zhenxin Wang
Journal:  Anal Sci       Date:  2008-07       Impact factor: 2.081

5.  Structure and function of nanoparticle-protein conjugates.

Authors:  M-E Aubin-Tam; K Hamad-Schifferli
Journal:  Biomed Mater       Date:  2008-08-08       Impact factor: 3.715

6.  Quantum dot bioconjugates for ultrasensitive nonisotopic detection.

Authors:  W C Chan; S Nie
Journal:  Science       Date:  1998-09-25       Impact factor: 47.728

7.  Semiconductor nanocrystals as fluorescent biological labels.

Authors:  M Bruchez; M Moronne; P Gin; S Weiss; A P Alivisatos
Journal:  Science       Date:  1998-09-25       Impact factor: 47.728

8.  Synthesis, stability, and cellular internalization of gold nanoparticles containing mixed peptide-poly(ethylene glycol) monolayers.

Authors:  Yanli Liu; Mathew K Shipton; Joseph Ryan; Eric D Kaufman; Stefan Franzen; Daniel L Feldheim
Journal:  Anal Chem       Date:  2007-02-09       Impact factor: 6.986

9.  Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Authors:  Raja Gopal Rayavarapu; Wilma Petersen; Constantin Ungureanu; Janine N Post; Ton G van Leeuwen; Srirang Manohar
Journal:  Int J Biomed Imaging       Date:  2007

10.  Applications of nanoparticles in biology and medicine.

Authors:  OV Salata
Journal:  J Nanobiotechnology       Date:  2004-04-30       Impact factor: 10.435
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  3 in total

1.  Cytotoxicity and Apoptosis Inducing Activities of 2-Amino-4H-chromene-3-carbonitrile Derivatives Loaded on Gold Nanoparticles Against Human Breast Cancer Cell Line T47D.

Authors:  Zahra Saffari; Maryam Farahnak Zarabi; Hasan Aryapour; Alireza Foroumadi; Ali Farhangi; Soheil Ghassemi; Azim Akbarzadeh
Journal:  Indian J Clin Biochem       Date:  2014-01-31

2.  In vitro antitumor evaluation of 4H-chromene-3-carbonitrile derivatives as a new series of apoptotic inducers.

Authors:  Zahra Saffari; Hassan Aryapour; Azim Akbarzadeh; Alireza Foroumadi; Najmeh Jafari; Maryam Farahnak Zarabi; Ali Farhangi
Journal:  Tumour Biol       Date:  2014-03-19

Review 3.  A Review on the Synthesis and Functionalization of Gold Nanoparticles as a Drug Delivery Vehicle.

Authors:  Sundus Jabeen Amina; Bin Guo
Journal:  Int J Nanomedicine       Date:  2020-12-07
  3 in total

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