| Literature DB >> 24750922 |
The Luong Nguyen1, Deyun Wang1, Yuanliang Hu1, Yunpeng Fan1, Junmin Wang1, Saifuding Abula1, Liwei Guo1, Jing Zhang1, Shem Kakai khakame2, Bao Khanh Dang3.
Abstract
The crude total Auricularia auricula polysaccharide (AAPct) was extracted by water decoction and ethanol precipitation, protein was removed to obtain total A. auricula polysaccharide (AAPt), then was graded into AAP1 and AAP2 through column chromatography. sAAPt, sAAP1 and sAAP2 were prepared by chlorosulfonic acid-pyridine method. In vitro test, the effects of sAAPt, sAAP1, sAAP2, AAPt, AAP1 and AAP2, on chicken peripheral lymphocytes proliferation were compared. The results showed that sAAPt and sAAP1 demonstrated better effect. In vivo test, 14-day-old chickens were injected respectively with sAAPt, sAAP1, AAPt and AAP1 at the first vaccination of ND vaccine, once a day for three days. On days 7, 14, 21 and 28 after the first vaccination, the peripheral lymphocytes proliferation and antibody titer were determined. The results indicated that sAAPt possessed the best efficacy and would be expected to be used as a component of a new-type immunopotentiator.Entities:
Mesh：
Substances：
Year: 2012 PMID： 24750922 DOI： 10.1016/j.carbpol.2012.03.082
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381