OBJECTIVES: The immune trypanolysis test (TL) is an accurate sero-diagnostic tool increasingly implemented for sleeping sickness medical surveillance, but it is restricted to the reference laboratories. To facilitate storage and transport of the test specimen, we developed a protocol for the examination of blood spotted on filter paper (TL-fp) that can be stored and shipped at ambient temperature. We compared its performance with the classical TL on plasma (TL-pl) that needs to be kept frozen until use. METHODS: The study was conducted in active foci of the Republic of Guinea. In total, 438 specimens from treated and untreated sleeping sickness patients and serological suspects were tested with both methods. RESULT: TL-fp gave significantly less positive results than TL-pl, but all the confirmed sleeping sickness cases were positive with the TL-fp protocol. CONCLUSION: TL-fp appears to offer a good compromise between feasibility and sensitivity to detect currently infected subjects who play a role in the transmission of Trypanosoma brucei gambiense and is useful for contributing to the elimination of gambiense sleeping sickness.
OBJECTIVES: The immune trypanolysis test (TL) is an accurate sero-diagnostic tool increasingly implemented for sleeping sickness medical surveillance, but it is restricted to the reference laboratories. To facilitate storage and transport of the test specimen, we developed a protocol for the examination of blood spotted on filter paper (TL-fp) that can be stored and shipped at ambient temperature. We compared its performance with the classical TL on plasma (TL-pl) that needs to be kept frozen until use. METHODS: The study was conducted in active foci of the Republic of Guinea. In total, 438 specimens from treated and untreated sleeping sicknesspatients and serological suspects were tested with both methods. RESULT: TL-fp gave significantly less positive results than TL-pl, but all the confirmed sleeping sickness cases were positive with the TL-fp protocol. CONCLUSION: TL-fp appears to offer a good compromise between feasibility and sensitivity to detect currently infected subjects who play a role in the transmission of Trypanosoma brucei gambiense and is useful for contributing to the elimination of gambiensesleeping sickness.
Keywords:
Tripanosomiasis Humana Africana; antibody; anticorps; anticuerpos; diagnosis; diagnostic; diagnóstico; filter paper; human African trypanosomiasis; immune trypanolysis test; papel de filtro; papier filtre; prueba de tripanólisis humana; surveillance; test de trypanolyse; trypanosomiase humaine africaine; vigilancia
Authors: Dieudonné Mumba Ngoyi; Rosine Ali Ekangu; Marie France Mumvemba Kodi; Patient Pati Pyana; Fatima Balharbi; Mélanie Decq; Victor Kande Betu; Wim Van der Veken; Claude Sese; Joris Menten; Philippe Büscher; Veerle Lejon Journal: PLoS Negl Trop Dis Date: 2014-06-12
Authors: Darshan Sudarshi; Sarah Lawrence; William Owen Pickrell; Vinay Eligar; Richard Walters; Shumonta Quaderi; Alice Walker; Paul Capewell; Caroline Clucas; Angela Vincent; Francesco Checchi; Annette MacLeod; Michael Brown Journal: PLoS Negl Trop Dis Date: 2014-12-18
Authors: Richard Selby; Charles Wamboga; Olema Erphas; Albert Mugenyi; Vincent Jamonneau; Charles Waiswa; Steve J Torr; Michael Lehane Journal: PLoS Negl Trop Dis Date: 2019-08-14
Authors: Raquel Inocencio da Luz; Sara Tablado Alonso; Philippe Büscher; Paul Verlé; Anja De Weggheleire; Dieudonné Mumba Ngoyi; Pati Patient Pyana; Epco Hasker Journal: Diagnostics (Basel) Date: 2022-01-19
Authors: Alexandre Girard; Anneli Cooper; Samuel Mabbott; Barbara Bradley; Steven Asiala; Lauren Jamieson; Caroline Clucas; Paul Capewell; Francesco Marchesi; Matthew P Gibbins; Franziska Hentzschel; Matthias Marti; Juan F Quintana; Paul Garside; Karen Faulds; Annette MacLeod; Duncan Graham Journal: PLoS Pathog Date: 2021-11-15 Impact factor: 6.823