BACKGROUND: Hepatic regeneration requires coordinated signal transduction for efficient restoration of functional liver mass. This study sought to determine changes in lysophosphatidic acid (LPA) and LPA receptor (LPAR) 1-6 expression in regenerating liver following two-thirds partial hepatectomy (PHx). METHODS: Liver tissue and blood were collected from male C57BL/6 mice following PHx. Circulating LPA was measured by enzyme-linked immunosorbent assay (ELISA) and hepatic LPAR mRNA and protein expression were determined. RESULTS: Circulating LPA increased 72 h after PHx and remained significantly elevated for up to 7 days post-PHx. Analysis of LPAR expression after PHx demonstrated significant increases in LPAR1, LPAR3 and LPAR6 mRNA and protein in a time-dependent manner for up to 7 days post-PHx. Conversely, LPAR2, LPAR4 and LPAR5 mRNA were barely detected in normal liver and did not significantly change after PHx. Changes in LPAR1 expression were confined to non-parenchymal cells following PHx. CONCLUSIONS: Liver regeneration following PHx is associated with significant changes in circulating LPA and hepatic LPAR1, LPAR3 and LPAR6 expression in a time- and cell-dependent manner. Furthermore, changes in LPA-LPAR post-PHx occur after the first round of hepatocyte division is complete.
BACKGROUND: Hepatic regeneration requires coordinated signal transduction for efficient restoration of functional liver mass. This study sought to determine changes in lysophosphatidic acid (LPA) and LPA receptor (LPAR) 1-6 expression in regenerating liver following two-thirds partial hepatectomy (PHx). METHODS: Liver tissue and blood were collected from male C57BL/6 mice following PHx. Circulating LPA was measured by enzyme-linked immunosorbent assay (ELISA) and hepatic LPAR mRNA and protein expression were determined. RESULTS: Circulating LPA increased 72 h after PHx and remained significantly elevated for up to 7 days post-PHx. Analysis of LPAR expression after PHx demonstrated significant increases in LPAR1, LPAR3 and LPAR6 mRNA and protein in a time-dependent manner for up to 7 days post-PHx. Conversely, LPAR2, LPAR4 and LPAR5 mRNA were barely detected in normal liver and did not significantly change after PHx. Changes in LPAR1 expression were confined to non-parenchymal cells following PHx. CONCLUSIONS: Liver regeneration following PHx is associated with significant changes in circulating LPA and hepatic LPAR1, LPAR3 and LPAR6 expression in a time- and cell-dependent manner. Furthermore, changes in LPA-LPAR post-PHx occur after the first round of hepatocyte division is complete.
Authors: M Yanase; H Ikeda; A Matsui; H Maekawa; E Noiri; T Tomiya; M Arai; T Yano; M Shibata; M Ikebe; K Fujiwara; M Rojkind; I Ogata Journal: Biochem Biophys Res Commun Date: 2000-10-14 Impact factor: 3.575
Authors: Stella M Mattaloni; Elena Kolobova; Cristián Favre; Raúl A Marinelli; James R Goldenring; Maria C Larocca Journal: J Cell Physiol Date: 2012-01 Impact factor: 6.384
Authors: H Ikeda; Y Yatomi; M Yanase; H Satoh; A Nishihara; M Kawabata; K Fujiwara Journal: Biochem Biophys Res Commun Date: 1998-07-20 Impact factor: 3.575
Authors: Eugene Sokolov; Ashley L Eheim; William A Ahrens; Tracy L Walling; Jacob H Swet; Matthew T McMillan; Kerri A Simo; Kyle J Thompson; David Sindram; Iain H McKillop Journal: J Surg Res Date: 2012-11-15 Impact factor: 2.192
Authors: Evan P Taddeo; Stefan R Hargett; Sujoy Lahiri; Marin E Nelson; Jason A Liao; Chien Li; Jill K Slack-Davis; Jose L Tomsig; Kevin R Lynch; Zhen Yan; Thurl E Harris; Kyle L Hoehn Journal: Sci Rep Date: 2017-03-09 Impact factor: 4.379
Authors: Valentina Zuckerman; Eugene Sokolov; Jacob H Swet; William A Ahrens; Victor Showlater; David A Iannitti; Iain H Mckillop Journal: Oncotarget Date: 2016-01-19