Literature DB >> 2475000

Identification of fetal hemoglobin in blood stains by high performance liquid chromatography.

H Inoue1, F Takabe, Y Maeno, M Iwasa.   

Abstract

A new method for the identification of fetal hemoglobin (Hb F) in blood stains by reverse-phase high performance liquid chromatography is described. Differentiation between fetal and adult blood stains is based on the existence of gamma-chain peaks which are characteristics of Hb F. Very few gamma chains appeared on chromatograms of all the adult blood stains examined. The level of Hb F could be determined by measuring the total of chromatogram gamma-globin chain areas, and expressing it as a percentage of total Hb. Levels in six cord blood stains on filter paper ranged from 81.1% to 91.3% and remained constant for at least 12 weeks. This method is of great value for its simplicity, sensitivity and speed, and most importantly for its reliability in the field of forensic medicine.

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Year:  1989        PMID： 2475000     DOI： 10.1007/bf00204074

Source DB:  PubMed          Journal:  Z Rechtsmed        ISSN： 0044-3433


  20 in total

1.  Significance of a new type of human fetal hemoglobin carrying a replacement isoleucine replaced by threonine at position 75 )E 19) of the gamma chain.

Authors:  G Ricco; U Mazza; R M Turi; P G Pich; C Camaschella; G Saglio; L F Bernini
Journal:  Hum Genet       Date:  1976-06-29       Impact factor: 4.132

Review 2.  Identification of characteristics in blood and semen stains--a review.

Authors:  I Oepen
Journal:  Forensic Sci Int       Date:  1988-02       Impact factor: 2.395

3.  The identification of foetal haemoglobin in bloodstains.

Authors:  B G Wraxall
Journal:  J Forensic Sci Soc       Date:  1972-07

4.  Prognostic value of fetal hemoglobin levels in acquired aplastic anemia.

Authors:  G E Bloom; L K Diamond
Journal:  N Engl J Med       Date:  1968-02-08       Impact factor: 91.245

5.  [Determination of foetal haemoglobin in bloodstains by means of electrophoresis on cellulose acetate (author's transl)].

Authors:  R Wilkens; I Oepen
Journal:  Z Rechtsmed       Date:  1977-03-23

6.  The detection of fetal hemoglobin in bloodstains by means of thin-layer immunoassay.

Authors:  E M Whitehead; M E Fredenburg; N T Lappas
Journal:  J Forensic Sci       Date:  1983-10       Impact factor: 1.832

7.  A new Hb variant: Hb F Sardinia gamma75(E19) isoleucine leads to threonine found in a family with Hb G Philadelphia, beta-chain deficiency and a Lepore-like haemoglobin indistinguishable from Hb A2.

Authors:  V Grifoni; H Kamuzora; H Lehmann; D Charlesworth
Journal:  Acta Haematol       Date:  1975       Impact factor: 2.195

8.  Minor (glycosylated) hemoglobins in cord blood of infants of normal and diabetic mothers.

Authors:  H E Fadel; A Reynolds; M Stallings; E C Abraham
Journal:  Am J Obstet Gynecol       Date:  1981-02-15       Impact factor: 8.661

9.  Glycosylated minor components of human fetal hemoglobin. Chromatographic separation, identification, and functional characterization.

Authors:  E C Abraham
Journal:  Biochim Biophys Acta       Date:  1981-01-30

10.  A new high-performance liquid chromatographic procedure to quantitate hemoglobin A1c and other minor hemoglobins in blood of normal, diabetic, and alcoholic individuals.

Authors:  T H Huisman; J B Henson; J B Wilson
Journal:  J Lab Clin Med       Date:  1983-08
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  3 in total

1.  Species identification of blood and bloodstains by high-performance liquid chromatography.

Authors:  H Inoue; F Takabe; O Takenaka; M Iwasa; Y Maeno
Journal:  Int J Legal Med       Date:  1990-12       Impact factor: 2.686

2.  Identification of fetal hemoglobin and simultaneous estimation of bloodstain age by high-performance liquid chromatography.

Authors:  H Inoue; F Takabe; M Iwasa; Y Maeno
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

Review 3.  Accuracy of Pulse Oximetry in the Presence of Fetal Hemoglobin-A Systematic Review.

Authors:  Ena Pritišanac; Berndt Urlesberger; Bernhard Schwaberger; Gerhard Pichler
Journal:  Children (Basel)       Date:  2021-04-30
  3 in total

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