Literature DB >> 2474629

Production and characterization of monoclonal antibodies specific for Mycobacterium bovis.

P R Wood1, J Ripper, A J Radford, P G Bundesen, D B Rylatt, L E Cottis, M John, P Plackett.   

Abstract

A series of monoclonal antibodies (MAbs), specific for Mycobacterium bovis and BCG strains, were tested extensively for cross-reactivity to a wide range of mycobacterial species using ELISA, Western blotting and dot-blot analysis. The MAbs bound specifically to M. bovis and BCG and showed limited cross-reactivity with some strains of M. tuberculosis. All these MAbs recognized a 22 kDa protein previously termed MPB70, and by competitive ELISA analysis appeared to detect at least three M. bovis-specific determinants on the MPB70 molecule.

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Year:  1988        PMID: 2474629     DOI: 10.1099/00221287-134-9-2599

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN: 0022-1287


  10 in total

1.  Use of polymerase chain reaction for rapid diagnosis of tuberculosis.

Authors:  D V Cousins; S D Wilton; B R Francis; B L Gow
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

2.  Modification of the Mycobacterium bovis extracellular protein MPB70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization.

Authors:  M Lin; E A Sugden; M E Jolley; K Stilwell
Journal:  Clin Diagn Lab Immunol       Date:  1996-07

3.  Crystalline cell surface layer of Mycobacterium bovis BCG.

Authors:  K Lounatmaa; E Brander
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

4.  Competitive and indirect enzyme-linked immunosorbent assays for Mycobacterium bovis infections based on MPB70 and lipoarabinomannan antigens.

Authors:  E A Sugden; K Stilwell; E B Rohonczy; P Martineau
Journal:  Can J Vet Res       Date:  1997-01       Impact factor: 1.310

5.  MPB70 and MPB83 as indicators of protein localization in mycobacterial cells.

Authors:  M Harboe; H G Wiker; G Ulvund; B Lund-Pedersen; A B Andersen; R G Hewinson; S Nagai
Journal:  Infect Immun       Date:  1998-01       Impact factor: 3.441

6.  Immunochemical characterization of the MPB70/80 and MPB83 proteins of Mycobacterium bovis.

Authors:  H G Wiker; K P Lyashchenko; A M Aksoy; K A Lightbody; J M Pollock; S V Komissarenko; S O Bobrovnik; I N Kolesnikova; L O Mykhalsky; M L Gennaro; M Harboe
Journal:  Infect Immun       Date:  1998-04       Impact factor: 3.441

7.  Production and evaluation of antibodies and phage display-derived peptide ligands for immunomagnetic separation of Mycobacterium bovis.

Authors:  Linda D Stewart; James McNair; Lyanne McCallan; Suzan Thompson; Leonid A Kulakov; Irene R Grant
Journal:  J Clin Microbiol       Date:  2012-02-08       Impact factor: 5.948

8.  Assessment of genetic markers for species differentiation within the Mycobacterium tuberculosis complex.

Authors:  E Liébana; A Aranaz; B Francis; D Cousins
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

9.  Purification and characterization of major antigens from a Mycobacterium bovis culture filtrate.

Authors:  T Fifis; C Costopoulos; A J Radford; A Bacic; P R Wood
Journal:  Infect Immun       Date:  1991-03       Impact factor: 3.441

10.  Identification of bovine T-cell epitopes for three Mycobacterium bovis antigens: MPB70, 19,000 MW and MPB57.

Authors:  J M Pollock; A J Douglas; D P Mackie; S D Neill
Journal:  Immunology       Date:  1994-05       Impact factor: 7.397

  10 in total

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