Johannes D Veldhuis1, Roy B Dyer2, Sergey A Trushin3, Olga P Bondar4, Ravinder J Singh4, George G Klee4. 1. Endocrine Research Unit, Mayo School of Graduate Medical Education, Mayo Clinic, Rochester, MN 55905. Electronic address: veldhuis.johannes@mayo.edu. 2. Immunochemical Laboratory, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905. 3. Immunochemical Laboratory, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905; Department of Neurology, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905. 4. Laboratory Medicine and Pathology, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905.
Abstract
OBJECTIVE: Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS). SETTING: Center for Translational Science Activities (CTSA). PARTICIPANTS: Healthy nonpregnant women (N=120) ages 21 to 79 years. OUTCOMES: SHBG, testosterone (T), estradiol (E2) and estrone (E1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids. RESULTS: By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P≤10(-4)), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P<10(-15), R(2)≥0.481). In addition, TAF and BMI were negatively associated with SHBG (P≤0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P≤0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P<10(-9), R(2)=0.358). CONCLUSION: According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.
OBJECTIVE:Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS). SETTING: Center for Translational Science Activities (CTSA). PARTICIPANTS: Healthy nonpregnant women (N=120) ages 21 to 79 years. OUTCOMES: SHBG, testosterone (T), estradiol (E2) and estrone (E1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids. RESULTS: By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P≤10(-4)), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P<10(-15), R(2)≥0.481). In addition, TAF and BMI were negatively associated with SHBG (P≤0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P≤0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P<10(-9), R(2)=0.358). CONCLUSION: According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.
Authors: L Sieminska; B Marek; B Kos-Kudla; D Niedziolka; D Kajdaniuk; M Nowak; J Glogowska-Szelag Journal: J Endocrinol Invest Date: 2004-06 Impact factor: 4.256
Authors: Johannes D Veldhuis; Dana Erickson; Kristi Mielke; Leon S Farhy; Daniel M Keenan; Cyril Y Bowers Journal: J Clin Endocrinol Metab Date: 2005-08-09 Impact factor: 5.958
Authors: Hylton V Joffe; Paul M Ridker; Joann E Manson; Nancy R Cook; Julie E Buring; Kathryn M Rexrode Journal: Ann Epidemiol Date: 2005-10-10 Impact factor: 3.797
Authors: Aimin Xu; Kok Weng Chan; Ruby L C Hoo; Yu Wang; Kathryn C B Tan; Jialiang Zhang; Baoying Chen; Michael C Lam; Cynthia Tse; Garth J S Cooper; Karen S L Lam Journal: J Biol Chem Date: 2005-03-09 Impact factor: 5.157
Authors: Rita Basu; Chiara Dalla Man; Marco Campioni; Ananda Basu; K Sree Nair; Michael D Jensen; Sundeep Khosla; George Klee; Gianna Toffolo; Claudio Cobelli; Robert A Rizza Journal: Diabetes Date: 2007-03 Impact factor: 9.461
Authors: P Lecomte; N Lecureuil; M Lecureuil; Y Lemonnier; N Mariotte; C Valat; M A Garrigue Journal: Eur J Endocrinol Date: 1998-08 Impact factor: 6.664
Authors: M Pugeat; P Moulin; P Cousin; S Fimbel; M H Nicolas; J C Crave; H Lejeune Journal: J Steroid Biochem Mol Biol Date: 1995-06 Impact factor: 4.292
Authors: Johannes D Veldhuis; Roy B Dyer; Sergey A Trushin; Olga P Bondar; Ravinder J Singh; George G Klee Journal: Endocrine Date: 2016-07-22 Impact factor: 3.633