Hayet Amalou1, Ayele H Negussie1, Ashish Ranjan1, Lucy Chow1, Sheng Xu1, Craig Kroeger2, Ziv Neeman3, Naomi P O'Grady4, Bradford J Wood5. 1. Center for Interventional Oncology, Clinical Center and National Cancer Institute, National Institutes of Health, Building 10, MSC 1182, Bethesda, MD 20892-1182. 2. VitalDyne, Inc, Cokato, Minnesota. 3. Center for Interventional Oncology, Clinical Center and National Cancer Institute, National Institutes of Health, Building 10, MSC 1182, Bethesda, MD 20892-1182; Radiological Associates, Philadelphia, Pennsylvania. 4. Critical Care Medicine Department, Clinical Center, National Institutes of Health, Building 10, MSC 1182, Bethesda, MD 20892-1182. 5. Center for Interventional Oncology, Clinical Center and National Cancer Institute, National Institutes of Health, Building 10, MSC 1182, Bethesda, MD 20892-1182. Electronic address: bwood@nih.gov.
Abstract
PURPOSE: To design, prototype, and assess a custom vascular access catheter for its ability to inhibit bacterial colonization in vitro and to optimize electric parameters for efficacy and safe translation. MATERIALS AND METHODS: A vascular access catheter with conductive elements was designed and custom fabricated with two electrodes at the tip, separated by a nonconductive segment. The catheter was colonized with Staphylococcus aureus and incubated at predetermined current levels (4-8 µA) and durations (4-24 h). Catheters were compared using bacterial counts and scanning electron microscopy (SEM). RESULTS: Bacteria colony-forming units were reduced significantly (P < .05) by > 90% (91.7%-100%) in all uninterrupted treatment arms that included electric current (4 µA or 8 µA) of at least 8 hours' duration. Qualitative analysis using SEM revealed that the treated catheter exposed to electric current had markedly less bacteria compared with the untreated catheter. CONCLUSIONS: This catheter with conductive elements inhibits bacterial colonization in vitro when very small electric current (4-8 µA) is applied across the tip for 8-24 hours. In vivo validation is requisite to future translation to the clinical setting. Published by Elsevier Inc.
PURPOSE: To design, prototype, and assess a custom vascular access catheter for its ability to inhibit bacterial colonization in vitro and to optimize electric parameters for efficacy and safe translation. MATERIALS AND METHODS: A vascular access catheter with conductive elements was designed and custom fabricated with two electrodes at the tip, separated by a nonconductive segment. The catheter was colonized with Staphylococcus aureus and incubated at predetermined current levels (4-8 µA) and durations (4-24 h). Catheters were compared using bacterial counts and scanning electron microscopy (SEM). RESULTS: Bacteria colony-forming units were reduced significantly (P < .05) by > 90% (91.7%-100%) in all uninterrupted treatment arms that included electric current (4 µA or 8 µA) of at least 8 hours' duration. Qualitative analysis using SEM revealed that the treated catheter exposed to electric current had markedly less bacteria compared with the untreated catheter. CONCLUSIONS: This catheter with conductive elements inhibits bacterial colonization in vitro when very small electric current (4-8 µA) is applied across the tip for 8-24 hours. In vivo validation is requisite to future translation to the clinical setting. Published by Elsevier Inc.
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