Literature DB >> 2474561

Quantitation of HIV-1 activity in tissue culture supernatants: effects of culture condition on syncytial assays and virus production.

G Spickett1, R E Beattie, L Bountiff, A G Dalgleish, A D Webster.   

Abstract

We have compared the infectivity titres, reverse transcriptase levels and antigen titres in the supernatants from persistently infected cell lines that produce a variety of HIV-1 isolates. We found a poor correlation between the different assays, and a variability in viral activity dependent on culture medium.

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Year:  1989        PMID： 2474561     DOI： 10.1016/0166-0934(89)90008-6

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  3 in total

1.  The effect of bioreactor configuration on production of HIV and cell-virus interaction.

Authors:  J B Clarke; J B Griffiths
Journal:  Cytotechnology       Date:  1990-09       Impact factor: 2.058

2.  Development of simian immunodeficiency virus isolation, titration, and neutralization assays which use whole blood from rhesus monkeys and an antigen capture enzyme-linked immunosorbent assay.

Authors:  B L Lohman; J Higgins; M L Marthas; P A Marx; N C Pedersen
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948

3.  Failure of ADCC to predict HIV-associated disease progression or outcome in a haemophiliac cohort.

Authors:  A Dalgleish; A Sinclair; M Steel; D Beatson; C Ludlam; J Habeshaw
Journal:  Clin Exp Immunol       Date:  1990-07       Impact factor: 4.330
  3 in total

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