| Literature DB >> 24743767 |
H Thierens1, A Vral2, C Vandevoorde2, V Vandersickel2, V de Gelder2, H Romm3, U Oestreicher3, K Rothkamm4, S Barnard4, E Ainsbury4, S Sommer5, C Beinke6, A Wojcik7.
Abstract
Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of the MN frequency distribution σ(2)/µ obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay.Mesh：
Year: 2014 PMID： 24743767 DOI： 10.1093/rpd/ncu130
Source DB: PubMed Journal: Radiat Prot Dosimetry ISSN： 0144-8420 Impact factor: 0.972