| Literature DB >> 24743043 |
Inna Chmelnitsky1, Maya Shklyar1, Azita Leavitt1, Evgeniya Sadovsky1, Shiri Navon-Venezia2, Maayan Ben Dalak1, Rotem Edgar1, Yehuda Carmeli3.
Abstract
We performed comparative sequence analysis of 3 blaKPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The blaKPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional blaKPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the blaKPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.Entities:
Keywords: Enterobacteriaceae; KPC; P2 promoter; Tn4401
Mesh：
Substances：
Year: 2014 PMID： 24743043 DOI： 10.1016/j.diagmicrobio.2014.03.008
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803