Berat Cem Özgür1, Onur Telli2, Cem Nedim Yuceturk2, Haşmet Sarici2, Elif Ozer3, Hatice Surer4, Aytun Sadan Kılınc4, Sema Hucumenoglu3, Muzaffer Eroglu2. 1. Urology Clinic, Ankara Research and Training Hospital, Ankara, Turkey. Electronic address: bcemozgur@hotmail.com. 2. Urology Clinic, Ankara Research and Training Hospital, Ankara, Turkey. 3. Pathology Department, Ankara Research and Training Hospital, Ankara, Turkey. 4. Biochemistry Department, Ankara Research and Training Hospital, Ankara, Turkey.
Abstract
PURPOSE: Ischemia-reperfusion injury can cause testicular damage and phosphodiesterase inhibitors are reported to regulate antioxidant activity. We investigated the prevention of ipsilateral and contralateral testicular damage using 2 phosphodiesterase inhibitors after testicular detorsion in rats. MATERIALS AND METHODS: A total of 28 adult male rats were randomly divided into 4 groups of 7 each, including group 1-sham operation, group 2-testicular torsion and detorsion, group 3- testicular torsion and detorsion with sildenafil administration before detorsion and group 4- testicular torsion and detorsion with udenafil administration before detorsion. Tissue levels of malondialdehyde, total sulfhydryl and nitrite were evaluated, and histopathological changes in the groups were examined. RESULTS: Compared to group 1 significantly increased tissue malondialdehyde (p = 0.001), significantly decreased total sulfhydryl (p = 0.038) and insignificantly increased nitrite were found in group 2. Compared to group 2 malondialdehyde decreased significantly and total sulfhydryl increased significantly in groups 3 and 4. The decrease in nitrite was insignificant in the latter 2 groups. Histopathology revealed increased hemorrhage, congestion and edema in group 2 rats. The testicular injury score was lower in groups 3 and 4. In group 2 grades II to IV injury was detected while most specimens in treated groups showed grade II injury. CONCLUSIONS: This study indicates that intraperitoneal administration of sildenafil and udenafil efficiently suppresses radical production while decreasing histological changes after testicular ischemia-reperfusion injury.
PURPOSE:Ischemia-reperfusion injury can cause testicular damage and phosphodiesterase inhibitors are reported to regulate antioxidant activity. We investigated the prevention of ipsilateral and contralateral testicular damage using 2 phosphodiesterase inhibitors after testicular detorsion in rats. MATERIALS AND METHODS: A total of 28 adult male rats were randomly divided into 4 groups of 7 each, including group 1-sham operation, group 2-testicular torsion and detorsion, group 3- testicular torsion and detorsion with sildenafil administration before detorsion and group 4- testicular torsion and detorsion with udenafil administration before detorsion. Tissue levels of malondialdehyde, total sulfhydryl and nitrite were evaluated, and histopathological changes in the groups were examined. RESULTS: Compared to group 1 significantly increased tissue malondialdehyde (p = 0.001), significantly decreased total sulfhydryl (p = 0.038) and insignificantly increased nitrite were found in group 2. Compared to group 2 malondialdehyde decreased significantly and total sulfhydryl increased significantly in groups 3 and 4. The decrease in nitrite was insignificant in the latter 2 groups. Histopathology revealed increased hemorrhage, congestion and edema in group 2 rats. The testicular injury score was lower in groups 3 and 4. In group 2 grades II to IV injury was detected while most specimens in treated groups showed grade II injury. CONCLUSIONS: This study indicates that intraperitoneal administration of sildenafil and udenafil efficiently suppresses radical production while decreasing histological changes after testicular ischemia-reperfusion injury.
Authors: Zulfu Sertkaya; Orhan Koca; Metin Ozturk; Mehmet Akyuz; Gulistan Gumrukcu; Musab Ali Kutluhan; Muhammet Ihsan Karaman Journal: Eurasian J Med Date: 2020-06-04
Authors: Ioannis D Kostakis; Nick Zavras; Christos Damaskos; Stratigoula Sakellariou; Penelope Korkolopoulou; Evangelos P Misiakos; Petros Tsaparas; George Vaos; Theodoros Karatzas Journal: Exp Ther Med Date: 2017-05-08 Impact factor: 2.447
Authors: Jong Mok Park; Ju Hyun Shin; Seung Woo Yang; Ji Yong Lee; Chung Lyul Lee; Jae Sung Lim; Ki Hak Song; Gun Hwa Kim; Yong Gil Na Journal: Int Neurourol J Date: 2021-09-03 Impact factor: 2.835