Ashok K Shenoy1, K V Ramesh1, Mukta N Chowta1, Prabha M Adhikari2, U P Rathnakar1. 1. Department of Pharmacology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India. 2. Department of Medicine, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
Abstract
OBJECTIVE: To evaluate the effects of botropase on various clotting factors in human volunteers. MATERIALS AND METHODS: It was a prospective open label study conducted on human healthy volunteers. After the baseline screening, subjects fulfilling inclusion criteria were enrolled. On the study day, 1 ml of botropase was administered intravenously and after an hour same dose of botropase (1 ml) was given by intramuscular (IM) route. The efficacy and safety parameters were monitored up to 72 h from the time of intravenous (IV) administration. RESULTS: A total of 15 volunteers, belonging to 24-35 years of age were included in the study. Botropase significantly reduced the plasma level of fibrinogen and fibrin degradation products after 5 min of IV administration (P < 0.05). In addition, factor X was observed to reduce constantly by botropase administration suggesting enhanced turnover between 5 and 20 min of IV administration. Although botropase reduced clotting and bleeding time in all the volunteers, the data remains to be statistically insignificant. CONCLUSION: Present study demonstrated the safety and efficacy of botropase in human healthy volunteers. The study has shown that it is a factor X activator and reduces effectively clotting and bleeding time.
OBJECTIVE: To evaluate the effects of botropase on various clotting factors in human volunteers. MATERIALS AND METHODS: It was a prospective open label study conducted on human healthy volunteers. After the baseline screening, subjects fulfilling inclusion criteria were enrolled. On the study day, 1 ml of botropase was administered intravenously and after an hour same dose of botropase (1 ml) was given by intramuscular (IM) route. The efficacy and safety parameters were monitored up to 72 h from the time of intravenous (IV) administration. RESULTS: A total of 15 volunteers, belonging to 24-35 years of age were included in the study. Botropase significantly reduced the plasma level of fibrinogen and fibrin degradation products after 5 min of IV administration (P < 0.05). In addition, factor X was observed to reduce constantly by botropase administration suggesting enhanced turnover between 5 and 20 min of IV administration. Although botropase reduced clotting and bleeding time in all the volunteers, the data remains to be statistically insignificant. CONCLUSION: Present study demonstrated the safety and efficacy of botropase in human healthy volunteers. The study has shown that it is a factor X activator and reduces effectively clotting and bleeding time.
Authors: N H Senden; T M Jeunhomme; J W Heemskerk; R Wagenvoord; C van't Veer; H C Hemker; W A Buurman Journal: J Immunol Date: 1998-10-15 Impact factor: 5.422