Literature DB >> 24740866

Single-molecule reconstruction of oligonucleotide secondary structure by atomic force microscopy.

Alice Pyne1, Ruth Thompson, Carl Leung, Debdulal Roy, Bart W Hoogenboom.   

Abstract

Based on soft-touch atomic force microscopy, a method is described to reconstruct the secondary structure of single extended biomolecules, without the need for crystallization. The method is tested by accurately reproducing the dimensions of the B-DNA crystal structure. Importantly, intramolecular variations in groove depth of the DNA double helix are resolved, which would be inaccessible for methods that rely on ensemble-averaging.
© 2014 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  DNA; atomic force microscopy; biomolecules; biophysics; oligonucleotides

Mesh：

Substances：

Year:  2014        PMID： 24740866     DOI： 10.1002/smll.201400265

Source DB:  PubMed          Journal:  Small        ISSN： 1613-6810            Impact factor:   13.281


  23 in total

1.  Bending and looping of long DNA by Polycomb repressive complex 2 revealed by AFM imaging in liquid.

Authors:  Patrick R Heenan; Xueyin Wang; Anne R Gooding; Thomas R Cech; Thomas T Perkins
Journal:  Nucleic Acids Res       Date:  2020-04-06       Impact factor: 16.971

2.  Atomic Force Microscopy of DNA and DNA-Protein Interactions.

Authors:  Philip J Haynes; Kavit H S Main; Bernice Akpinar; Alice L B Pyne
Journal:  Methods Mol Biol       Date:  2022

3.  Immunoactivity of self-assembled antibodies investigated by atomic force microscopy.

Authors:  Hiroaki Kominami; Kei Kobayashi; Shinichiro Ido; Hirokazu Kimiya; Hirofumi Yamada
Journal:  RSC Adv       Date:  2018-08-20       Impact factor: 4.036

4.  Design, assembly, and characterization of membrane-spanning DNA nanopores.

Authors:  Conor Lanphere; Daniel Offenbartl-Stiegert; Adam Dorey; Genevieve Pugh; Elena Georgiou; Yongzheng Xing; Jonathan R Burns; Stefan Howorka
Journal:  Nat Protoc       Date:  2020-12-21       Impact factor: 13.491

Review 5.  Novel imaging technologies for characterization of microbial extracellular polysaccharides.

Authors:  Magnus B Lilledahl; Bjørn T Stokke
Journal:  Front Microbiol       Date:  2015-05-28       Impact factor: 5.640

6.  Disruption of Higher Order DNA Structures in Friedreich's Ataxia (GAA)n Repeats by PNA or LNA Targeting.

Authors:  Helen Bergquist; Cristina S J Rocha; Rubén Álvarez-Asencio; Chi-Hung Nguyen; Mark W Rutland; C I Edvard Smith; Liam Good; Peter E Nielsen; Rula Zain
Journal:  PLoS One       Date:  2016-11-15       Impact factor: 3.240

7.  Novel approach for label free super-resolution imaging in far field.

Authors:  Sergey A Alexandrov; James McGrath; Hrebesh Subhash; Francesca Boccafoschi; Cinzia Giannini; Martin Leahy
Journal:  Sci Rep       Date:  2015-09-03       Impact factor: 4.379

8.  Glass is a Viable Substrate for Precision Force Microscopy of Membrane Proteins.

Authors:  Nagaraju Chada; Krishna P Sigdel; Raghavendar Reddy Sanganna Gari; Tina Rezaie Matin; Linda L Randall; Gavin M King
Journal:  Sci Rep       Date:  2015-07-31       Impact factor: 4.379

9.  Atomic Force Microscopy Protocol for Measurement of Membrane Plasticity and Extracellular Interactions in Single Neurons in Epilepsy.

Authors:  Xin Wu; Mariappan Muthuchamy; Doodipala Samba Reddy
Journal:  Front Aging Neurosci       Date:  2016-05-04       Impact factor: 5.750

10.  Accurate nanoscale flexibility measurement of DNA and DNA-protein complexes by atomic force microscopy in liquid.

Authors:  Divakaran Murugesapillai; Serge Bouaziz; L James Maher; Nathan E Israeloff; Craig E Cameron; Mark C Williams
Journal:  Nanoscale       Date:  2017-08-10       Impact factor: 7.790
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