Literature DB >> 24740549

Analysis of cytosolic pH changes in thymocytes during early apoptosis with improved three-channel real-time fluorescence imaging.

Zhang Shu1, Liu Xiaochen, Deng Chuyun, Han Man, Pan Juhua, Liao Xinghua, Qi Xin, Duan Shaojin, Ma Wanyun.   

Abstract

Cytosolic pH changes in single thymocytes in the early stages of apoptosis were examined with spectral-crosstalk-improved three-channel real-time fluorescence imaging. Spectral crosstalk was greatly reduced by this improved image processing method. Compared with normal thymocytes, the improved image processing method showed that the cytosolic pH value of thymocytes undergoing apoptosis was lower. A slight diversity in the intracellular pH values of samples subjected to different drug treatments was also observed. This new image processing method is beneficial to the application of the three-channel real-time fluorescence imaging to biological processes.

Mesh：

Year:  2014        PMID： 24740549     DOI： 10.1007/s10895-014-1384-1

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  18 in total

1.  Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy.

Authors:  Masilamani Elangovan; Horst Wallrabe; Ye Chen; Richard N Day; Margarida Barroso; Ammasi Periasamy
Journal:  Methods       Date:  2003-01       Impact factor: 3.608

2.  Assessing protein-surface interactions with a series of multi-labeled BSA using fluorescence lifetime microscopy and Förster Energy Resonance Transfer.

Authors:  Denisio M Togashi; Alan G Ryder
Journal:  Biophys Chem       Date:  2010-08-03       Impact factor: 2.352

3.  Micronucleus formation detected by live-cell imaging.

Authors:  Yun Huang; Michael Fenech; Qinghua Shi
Journal:  Mutagenesis       Date:  2011-01       Impact factor: 3.000

4.  Real time, high resolution video imaging of apoptosis in single cells with a polymeric nanoprobe.

Authors:  Seulki Lee; Ki Young Choi; Hyunjin Chung; Ju Hee Ryu; Aeju Lee; Heebeom Koo; In-Chan Youn; Jae Hyung Park; In-San Kim; Sang Yoon Kim; Xiaoyuan Chen; Seo Young Jeong; Ick Chan Kwon; Kwangmeyung Kim; Kuiwon Choi
Journal:  Bioconjug Chem       Date:  2011-01-10       Impact factor: 4.774

5.  Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes.

Authors:  Hari Shroff; Catherine G Galbraith; James A Galbraith; Helen White; Jennifer Gillette; Scott Olenych; Michael W Davidson; Eric Betzig
Journal:  Proc Natl Acad Sci U S A       Date:  2007-12-12       Impact factor: 11.205

6.  Application of a new pH-sensitive fluoroprobe (carboxy-SNARF-1) for intracellular pH measurement in small, isolated cells.

Authors:  K J Buckler; R D Vaughan-Jones
Journal:  Pflugers Arch       Date:  1990-10       Impact factor: 3.657

7.  Real-time imaging of viable-apoptotic switch in GSNO-induced mouse thymocyte apoptosis.

Authors:  Dan-ying Lin; Wan-yun Ma; Shao-jin Duan; Yan Zhang; Li-ying Du
Journal:  Apoptosis       Date:  2006-08       Impact factor: 4.677

8.  Visualization of Cu²⁺ uptake and release in plant cells by fluorescence lifetime imaging microscopy.

Authors:  Benjamin Hötzer; Rumen Ivanov; Tzvetina Brumbarova; Petra Bauer; Gregor Jung
Journal:  FEBS J       Date:  2011-12-19       Impact factor: 5.542

9.  SNARF-1 as an intracellular pH indicator in laser microspectrofluorometry: a critical assessment.

Authors:  O Seksek; N Henry-Toulmé; F Sureau; J Bolard
Journal:  Anal Biochem       Date:  1991-02-15       Impact factor: 3.365

10.  Measurement of intracellular pH using flow cytometry with carboxy-SNARF-1.

Authors:  E D Wieder; H Hang; M H Fox
Journal:  Cytometry       Date:  1993-11
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