Guan Xiao Ying, L I Wen Sheng1, Zhang Li Xia, Wang Hong Tao. 1. Department of Pathology, Shaanxi Provincial People's Hospital, Third Affiliated Hospital of the School of Medicine, Xi'an Jiaotong University, Xi'an, 710068, China.
Abstract
BACKGROUND: The relationship between Helicobacter pylori (Hp) infection and the risk of esophageal squamous cell carcinoma is unclear. AIM: The purpose was to investigate the effects of CagA+ Hp on esophageal squamous carcinoma Ec 109 cells in vitro and explore the molecular mechanisms underlying these effects. MATERIALS AND METHODS: Ec 109 cells were treated with CagA+ Hp filtrate at a concentration of 1.0 mg/mL or 50 μg/mL in vitro, proliferation and apoptosis of Ec 109 cells were assayed, the secretion of IL-8 was measured by ELISA, and the levels of Src homology-2 domain-containing phosphatase (SHP-2) mRNAs was assayed by RT-PCR.. Furthermore, after pretreatment of Ec109 cells with the specific p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580, the p38 pathway was detected. RESULTS: CagA+ Hp filtrate enhanced both the proliferation and apoptosis of Ec 109 cells. In addition, cytokine IL-8 release was significantly increased, and the expression of SHP-2 mRNA declined sharply in the CagA+ Hp group. Furthermore, after pretreatment of Ec109 cells with the specific p38 MAPK inhibitor SB203580, Ec109 cells proliferation and IL-8 secretion were inhibited. CONCLUSIONS: Our results suggest that CagA+ Hp filtrates could induce proliferation and the secretion of IL-8 by Ec109 cells in vitro. IL-8 secretion was induced through the activation of the p38 MAPK signal pathway.
BACKGROUND: The relationship between Helicobacter pylori (Hp) infection and the risk of esophageal squamous cell carcinoma is unclear. AIM: The purpose was to investigate the effects of CagA+ Hp on esophageal squamous carcinoma Ec 109 cells in vitro and explore the molecular mechanisms underlying these effects. MATERIALS AND METHODS: Ec 109 cells were treated with CagA+ Hp filtrate at a concentration of 1.0 mg/mL or 50 μg/mL in vitro, proliferation and apoptosis of Ec 109 cells were assayed, the secretion of IL-8 was measured by ELISA, and the levels of Src homology-2 domain-containing phosphatase (SHP-2) mRNAs was assayed by RT-PCR.. Furthermore, after pretreatment of Ec109 cells with the specific p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580, the p38 pathway was detected. RESULTS:CagA+ Hp filtrate enhanced both the proliferation and apoptosis of Ec 109 cells. In addition, cytokine IL-8 release was significantly increased, and the expression of SHP-2 mRNA declined sharply in the CagA+ Hp group. Furthermore, after pretreatment of Ec109 cells with the specific p38 MAPK inhibitor SB203580, Ec109 cells proliferation and IL-8 secretion were inhibited. CONCLUSIONS: Our results suggest that CagA+ Hp filtrates could induce proliferation and the secretion of IL-8 by Ec109 cells in vitro. IL-8 secretion was induced through the activation of the p38 MAPK signal pathway.