Xiaoling Li1, Wenhui Liu1, Fei Feng1, Xiaoqin Hu1, Ping Yuan1, Jin Yan2, Yanfang Yang3. 1. Department of Epidemiology, West China School of Public Health, Sichuan University, Chengdu 610041, China. 2. Department of Intestinal Surgery, Sichuan Cancer Hospital. 3. Department of Epidemiology, West China School of Public Health, Sichuan University, Chengdu 610041, China. Email: yang2009@scu.edu.cn.
Abstract
OBJECTIVE: To explore the association between adiponectin rs2241766, rs1501299 polymorphisms and gene-environment interaction and the risk of colorectal cancer(CRC). METHODS: Four hundred CRC patients confirmed by histopathology and 400 healthy controls were recruited in this study. Cases and controls were matched on age and gender. A well-designed questionnaire was used to collect the information of demography, lifestyle and dietary habit on the 400 case-control pairs. Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was applied to detect the adiponectin rs2241766 and rs1501299 genotypes. RESULTS: Data from conditional logistic regression analysis showed that those carrying TG+GG genotype on rs2241766 having an increased risk compared to those that carrying TT genotype(OR = 1.354, 95%CI:1.004-1.827), and those that carrying GT+TT genotype on rs1501299 having an decreased risk when compared to those that carrying GG genotype(OR = 0.680, 95% CI:0.501-0.923), after adjusted by factors as CRC family history,BMI, sedentary time, red meat consumption, and tea-drinking habit. Data from generalized multifactor dimensionality reduction showed that the gene-environment interaction among rs2241766, rs1501299 and red meat consumption on the risk of CRC might be significant(P = 0.001). A significant dosage effect with an increasing number of risk genotypes was observed as the risk of CRC increased (χ(2) = 8.458, P = 0.004). CONCLUSION: Both adiponectin rs2241766 and rs1501299 were associated with CRC risk and the two SNPs might have worked together with red meat consumption in affecting the CRC risk.
OBJECTIVE: To explore the association between adiponectinrs2241766, rs1501299 polymorphisms and gene-environment interaction and the risk of colorectal cancer(CRC). METHODS: Four hundred CRC patients confirmed by histopathology and 400 healthy controls were recruited in this study. Cases and controls were matched on age and gender. A well-designed questionnaire was used to collect the information of demography, lifestyle and dietary habit on the 400 case-control pairs. Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was applied to detect the adiponectinrs2241766 and rs1501299 genotypes. RESULTS: Data from conditional logistic regression analysis showed that those carrying TG+GG genotype on rs2241766 having an increased risk compared to those that carrying TT genotype(OR = 1.354, 95%CI:1.004-1.827), and those that carrying GT+TT genotype on rs1501299 having an decreased risk when compared to those that carrying GG genotype(OR = 0.680, 95% CI:0.501-0.923), after adjusted by factors as CRC family history,BMI, sedentary time, red meat consumption, and tea-drinking habit. Data from generalized multifactor dimensionality reduction showed that the gene-environment interaction among rs2241766, rs1501299 and red meat consumption on the risk of CRC might be significant(P = 0.001). A significant dosage effect with an increasing number of risk genotypes was observed as the risk of CRC increased (χ(2) = 8.458, P = 0.004). CONCLUSION: Both adiponectinrs2241766 and rs1501299 were associated with CRC risk and the two SNPs might have worked together with red meat consumption in affecting the CRC risk.