Asuncion Mejias1, Nicolas M Suarez, Octavio Ramilo. 1. Division of Pediatric Infectious Diseases, Center for Vaccines and Immunity, Nationwide Children's Hospital, The Ohio State University, Columbus, Ohio, USA.
Abstract
PURPOSE OF REVIEW: There is a need for improved diagnosis and for optimal classification of patients with infectious diseases. An alternative approach to the pathogen-detection strategy is based on a comprehensive analysis of the host response to the infection. This review focuses on the value of transcriptome analyses of blood leukocytes for the diagnosis and management of patients with infectious diseases. RECENT FINDINGS: Initial studies showed that RNA from blood leukocytes of children with acute viral and bacterial infections carried pathogen-specific transcriptional signatures. Subsequently, transcriptional signatures for several other infections have been described and validated in humans with malaria, dengue, salmonella, melioidosis, respiratory syncytial virus, influenza, tuberculosis, and HIV. In addition, transcriptome analyses represent an invaluable tool to understand disease pathogenesis and to objectively classify patients according to the clinical severity. SUMMARY: Microarray studies have been shown to be highly reproducible using different platforms, and in different patient populations, confirming the value of blood transcriptome analyses to study pathogen-specific host immune responses in the clinical setting. Combining the detection of the pathogen with a comprehensive assessment of the host immune response will provide a new understanding of the correlations between specific causative agents, the host response, and the clinical manifestations of the disease.
PURPOSE OF REVIEW: There is a need for improved diagnosis and for optimal classification of patients with infectious diseases. An alternative approach to the pathogen-detection strategy is based on a comprehensive analysis of the host response to the infection. This review focuses on the value of transcriptome analyses of blood leukocytes for the diagnosis and management of patients with infectious diseases. RECENT FINDINGS: Initial studies showed that RNA from blood leukocytes of children with acute viral and bacterial infections carried pathogen-specific transcriptional signatures. Subsequently, transcriptional signatures for several other infections have been described and validated in humans with malaria, dengue, salmonella, melioidosis, respiratory syncytial virus, influenza, tuberculosis, and HIV. In addition, transcriptome analyses represent an invaluable tool to understand disease pathogenesis and to objectively classify patients according to the clinical severity. SUMMARY: Microarray studies have been shown to be highly reproducible using different platforms, and in different patient populations, confirming the value of blood transcriptome analyses to study pathogen-specific host immune responses in the clinical setting. Combining the detection of the pathogen with a comprehensive assessment of the host immune response will provide a new understanding of the correlations between specific causative agents, the host response, and the clinical manifestations of the disease.
Authors: Jennifer C Boldrick; Ash A Alizadeh; Maximilian Diehn; Sandrine Dudoit; Chih Long Liu; Christopher E Belcher; David Botstein; Louis M Staudt; Patrick O Brown; David A Relman Journal: Proc Natl Acad Sci U S A Date: 2002-01-22 Impact factor: 11.205
Authors: Damien Chaussabel; Roshanak Tolouei Semnani; Mary Ann McDowell; David Sacks; Alan Sher; Thomas B Nutman Journal: Blood Date: 2003-03-27 Impact factor: 22.113
Authors: Li Liu; Hope L Johnson; Simon Cousens; Jamie Perin; Susana Scott; Joy E Lawn; Igor Rudan; Harry Campbell; Richard Cibulskis; Mengying Li; Colin Mathers; Robert E Black Journal: Lancet Date: 2012-05-11 Impact factor: 79.321
Authors: Matthew P R Berry; Christine M Graham; Finlay W McNab; Zhaohui Xu; Susannah A A Bloch; Tolu Oni; Katalin A Wilkinson; Romain Banchereau; Jason Skinner; Robert J Wilkinson; Charles Quinn; Derek Blankenship; Ranju Dhawan; John J Cush; Asuncion Mejias; Octavio Ramilo; Onn M Kon; Virginia Pascual; Jacques Banchereau; Damien Chaussabel; Anne O'Garra Journal: Nature Date: 2010-08-19 Impact factor: 49.962
Authors: Feng Qian; Lisa Chung; Wei Zheng; Vincent Bruno; Roger P Alexander; Zhong Wang; Xiaomei Wang; Sebastian Kurscheid; Hongyu Zhao; Erol Fikrig; Mark Gerstein; Michael Snyder; Ruth R Montgomery Journal: Viruses Date: 2013-07-08 Impact factor: 5.048
Authors: Amy C Shurtleff; Chris A Whitehouse; Michael D Ward; Lisa H Cazares; Sina Bavari Journal: Front Microbiol Date: 2015-02-20 Impact factor: 5.640
Authors: Jacqueline M Cliff; Stefan H E Kaufmann; Helen McShane; Paul van Helden; Anne O'Garra Journal: Immunol Rev Date: 2015-03 Impact factor: 12.988
Authors: Kun Wang; Stanley Langevin; Corey S O'Hern; Mark D Shattuck; Serenity Ogle; Adriana Forero; Juliet Morrison; Richard Slayden; Michael G Katze; Michael Kirby Journal: PLoS One Date: 2016-08-17 Impact factor: 3.240
Authors: Jingjing Zhang; Ida M Friberg; Ann Kift-Morgan; Gita Parekh; Matt P Morgan; Anna Rita Liuzzi; Chan-Yu Lin; Kieron L Donovan; Chantal S Colmont; Peter H Morgan; Paul Davis; Ian Weeks; Donald J Fraser; Nicholas Topley; Matthias Eberl Journal: Kidney Int Date: 2017-03-17 Impact factor: 10.612
Authors: Esther Bartholomeus; Nicolas De Neuter; Annelies Lemay; Luc Pattyn; David Tuerlinckx; David Weynants; Koen Van Lede; Gerlant van Berlaer; Dominique Bulckaert; Tine Boiy; Ann Vander Auwera; Marc Raes; Dimitri Van der Linden; Helene Verhelst; Susanne Van Steijn; Tijl Jonckheer; Joke Dehoorne; Rik Joos; Hilde Jansens; Arvid Suls; Pierre Van Damme; Kris Laukens; Geert Mortier; Pieter Meysman; Benson Ogunjimi Journal: J Transl Med Date: 2019-08-23 Impact factor: 5.531
Authors: Lauri Ivaska; Jussi Niemelä; Johanna Lempainen; Riikka Österback; Matti Waris; Tytti Vuorinen; Jukka Hytönen; Kaisu Rantakokko-Jalava; Ville Peltola Journal: J Infect Date: 2017-01-07 Impact factor: 6.072