Literature DB >> 24739157

Measuring microtubule polarity in spindles with second-harmonic generation.

Che-Hang Yu1, Noah Langowitz2, Hai-Yin Wu3, Reza Farhadifar4, Jan Brugues5, Tae Yeon Yoo2, Daniel Needleman4.   

Abstract

The spatial organization of microtubule polarity, and the interplay between microtubule polarity and protein localization, is thought to be crucial for spindle assembly, anaphase, and cytokinesis, but these phenomena remain poorly understood, in part due to the difficulty of measuring microtubule polarity in spindles. We develop and implement a method to nonperturbatively and quantitatively measure microtubule polarity throughout spindles using a combination of second-harmonic generation and two-photon fluorescence. We validate this method using computer simulations and by comparison to structural data on spindles obtained from electron tomography and laser ablation. This method should provide a powerful tool for studying spindle organization and function, and may be applicable for investigating microtubule polarity in other systems.
Copyright © 2014 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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Year:  2014        PMID： 24739157      PMCID： PMC4008835          DOI： 10.1016/j.bpj.2014.03.009

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  59 in total

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  11 in total

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