Literature DB >> 2473632

Enhancement of the histamine releasing activity of mouse monoclonal anti-human IgE antibody xb6-16 when present in aggregated or complexed forms.

A Weyer1, J P Dandeu, M Etiévant, J P Bourgeois, F Marchand, A Prouvost-Danon, B David.   

Abstract

FPLC purification of mouse monoclonal anti-human IgE antibody xb6-16 showed 2 major peaks of different molecular weight, peak 1 (greater than 10(6) d) and peak 3 (1.6 x 10(5) d). Peak 1 consisted of IgG1 and IgM, peak 3 of IgG1 only. On a protein weight basis, peak 1 was 100 times more potent than peak 3 in inducing histamine release from human basophils. Preincubation of peak 3 with anti-IgG1 enhanced the mediator release triggered by this fraction. On this basis, the potentiating effect of aggregated IgG1 or IgG1-IgM complexes on mediator release from basophils is discussed.

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Year:  1989        PMID： 2473632     DOI： 10.1007/bf02222196

Source DB:  PubMed          Journal:  Agents Actions        ISSN： 0065-4299


  7 in total

1.  Characteristics of histamine release from rat mast cells in relation to the valency of the stimulating ligand.

Authors:  R M Healicon; J C Foreman
Journal:  Immunology       Date:  1986-04       Impact factor: 7.397

2.  Passive in vitro sensitization of human basophils with Dermatophagoides farinae specific IgE.

Authors:  A Weyer; P Ougen; J P Dandeu; F Marchand; B David
Journal:  Agents Actions       Date:  1986-04

3.  Basophil sensitivity and reactivity to monoclonal anti-human IgE after in vitro sensitization with human myeloma IgE.

Authors:  A Weyer; J L Guesdon; P Ougen; F Marchand; B David
Journal:  Agents Actions       Date:  1987-04

4.  Monoclonal anti-human IgE: histamine release capacity and effect on in vitro sensitization of human basophils.

Authors:  A Weyer; C Demeulemester; J C Mazie; G Peltre; F Marchand; B David
Journal:  Agents Actions       Date:  1987-04

5.  A high-sampling-rate automated continuous-flow fluorometric technique for the analysis of nanogram levels of histamine in biological samples.

Authors:  B Lebel
Journal:  Anal Biochem       Date:  1983-08       Impact factor: 3.365

6.  Studies on Dermatophagoides pteronyssinus allergens: measurement of the relative potencies of D. pteronyssinus purified extracts by in vitro and in vivo methods.

Authors:  J Le Mao; A Weyer; G Pauli; B Lebel; B David
Journal:  J Allergy Clin Immunol       Date:  1980-05       Impact factor: 10.793

7.  Monoclonal antibodies to human IgE: utilization for total IgE quantification and estimation of allergen specific IgE antibodies.

Authors:  J P Bourgeois; M Père; J C Mazié; J F Delagneau
Journal:  Dev Biol Stand       Date:  1984
  7 in total

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