D Souna1, A S Amir2, S N Bekhoucha3, M Berrazeg4, M Drissi4. 1. Laboratoire antibiotiques, antifongiques, physico-chimie, synthèse et activité biologiques (LAPSAB), faculté des sciences de la nature, de la vie, de la terre et de l'univers, université Abou-Bekr-Belkaid-Tlemcen, BP 119, Tlemcen 13000, Algeria. Electronic address: souna.microbiologie@yahoo.fr. 2. Service d'anesthésie-réanimation du CHU Hassani-Abdelkader-Sidi, Bel-Abbes, Algeria. 3. Laboratoire de microbiologie du CHU d'Oran, rue du Dr Benzerdjeb-Oran, Oran, Algeria. 4. Laboratoire antibiotiques, antifongiques, physico-chimie, synthèse et activité biologiques (LAPSAB), faculté des sciences de la nature, de la vie, de la terre et de l'univers, université Abou-Bekr-Belkaid-Tlemcen, BP 119, Tlemcen 13000, Algeria.
Abstract
OBJECTIVES: Enterobacter cloacae is a major nosocomial bacterium causing severe infections. A multicenter retrospective cohort study was conducted to collect baseline information on the molecular characteristics of β-lactamase producing Enterobacter cloacae in the west of Algeria. MATERIALS AND METHODS: We report a series of 42 extended-spectrum β-lactamase (ESBL) producing non-repetitive Enterobacter cloacae strains, collected in 3 university hospital (Tlemcen, Oran, and Sidi Bel Abbes). Antibiotic susceptibility testing (antibiogram and MIC) and screening for ESBL were performed according to the French Society for Microbiology guidelines. PFGE typing was used to characterize the clonality of all the strains. β-lactamase genes (blaTEM, blaSHV, blaCTX-M, blaECB, and blaCMY-2) were amplified by PCR with specific primers. Plasmid isolation, electroporation, and conjugation experiments were carried out using standard methods. RESULTS: Sequence analysis revealed that most strains produced CTX-M type ESBLs (CTX-M-15 and CTX-M-3), whereas only 5 produced SHV-type ESBLs (SHV-12). The blaTEM gene was identified in all strains of Enterobacter cloacae. Several epidemic clones were determined. One strain was found to produce plasmid-mediated AmpC β-lactamase (CMY-2); this gene was transferred from E. cloacae by electroporation. Conjugation experiments showed that blaCTX-M, blaTEM, and blaSHV were carried by conjugative plasmids of high molecular weight (≥70kb). CONCLUSION: The emergence of resistance genes is a public health problem.
OBJECTIVES:Enterobacter cloacae is a major nosocomial bacterium causing severe infections. A multicenter retrospective cohort study was conducted to collect baseline information on the molecular characteristics of β-lactamase producing Enterobacter cloacae in the west of Algeria. MATERIALS AND METHODS: We report a series of 42 extended-spectrum β-lactamase (ESBL) producing non-repetitive Enterobacter cloacae strains, collected in 3 university hospital (Tlemcen, Oran, and Sidi Bel Abbes). Antibiotic susceptibility testing (antibiogram and MIC) and screening for ESBL were performed according to the French Society for Microbiology guidelines. PFGE typing was used to characterize the clonality of all the strains. β-lactamase genes (blaTEM, blaSHV, blaCTX-M, blaECB, and blaCMY-2) were amplified by PCR with specific primers. Plasmid isolation, electroporation, and conjugation experiments were carried out using standard methods. RESULTS: Sequence analysis revealed that most strains produced CTX-M type ESBLs (CTX-M-15 and CTX-M-3), whereas only 5 produced SHV-type ESBLs (SHV-12). The blaTEM gene was identified in all strains of Enterobacter cloacae. Several epidemic clones were determined. One strain was found to produce plasmid-mediated AmpC β-lactamase (CMY-2); this gene was transferred from E. cloacae by electroporation. Conjugation experiments showed that blaCTX-M, blaTEM, and blaSHV were carried by conjugative plasmids of high molecular weight (≥70kb). CONCLUSION: The emergence of resistance genes is a public health problem.
Authors: Frieder Schaumburg; Abraham S Alabi; Lisa Frielinghaus; Martin P Grobusch; Robin Köck; Karsten Becker; Saadou Issifou; Peter G Kremsner; Georg Peters; Alexander Mellmann Journal: BMC Microbiol Date: 2014-11-19 Impact factor: 3.605