Literature DB >> 24731359

Structural elucidation and cellular antioxidant activity evaluation of major antioxidant phenolics in lychee pulp.

Dongxiao Su1, Huihui Ti2, Ruifen Zhang2, Mingwei Zhang3, Zhengchen Wei2, Yuanyuan Deng2, Jinxin Guo2.   

Abstract

Lychee pulp contains phenolic compounds that are strong antioxidants, but the identities of the major antioxidants present are unknown. In the present study, the major contributors to the antioxidant activity of fresh lychee pulp were identified and their cellular antioxidant activities were investigated. Aqueous acetone extracts of lychee pulp were fractionated on polyamide resin, and those fractions with the largest antioxidant and radical scavenging activities were selected using cellular antioxidant activity and oxygen radical absorbance capacity assays. Three compounds that were major contributors to the antioxidant activity in these fractions were obtained by reverse-phase preparative HPLC and identified as quercetin 3-O-rutinoside-7-O-α-L-rhamnosidase (quercetin 3-rut-7-rha), quercetin 3-O-rutinoside (rutin) and (-)-epicatechin using NMR spectroscopy, HMBC, and ESI-MS spectrometry. The concentration of quercetin 3-rut-7-rha was 17.25mg per 100g of lychee pulp fresh weight. This is the first report of the identification and cellular antioxidant activity of quercetin 3-rut-7-rha from lychee pulp.
Copyright © 2014 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Antioxidant activity; Cellular antioxidant activity; Lychee; Oxygen radical absorbance capacity; Phenolics; Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase

Mesh:

Substances:

Year:  2014        PMID: 24731359     DOI: 10.1016/j.foodchem.2014.02.134

Source DB:  PubMed          Journal:  Food Chem        ISSN: 0308-8146            Impact factor:   7.514


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