Literature DB >> 2473053

Detection of granulocyte colony-stimulating factor produced by a newly established human hepatoma cell line using a simple bioassay system.

K Tohyama1, Y Yoshida, A Kubo, T Sudo, M Moriyama, H Sato, H Uchino.   

Abstract

A colony-stimulating factor(CSF)-producing tumor cell line (KX-87) was established from a patient with hepatocellular carcinoma and marked granulocytosis. This cell line formed tumors on nude mice in high frequency and the mice revealed marked granulocytosis. In clonogenic assays of human bone marrow cells, KX-87 conditioned medium (CM) supported the formation of colonies mainly consisting of neutrophilic granulocytes but had no burst-promoting activity. The molecular weight of the colony-stimulating activity (CSA) in KX-87CM was estimated about 25,000 daltons by gel filtration and a new bioassay system. In principle, a subline of murine hemopoietic cell line NFS-60 was cloned which was dependent on KX-87CM. Then the growth of this subline was examined by a rapid and sensitive colorimetric tetrazolium assay. From these results, it was concluded that the CSA which KX-87 cell line produced was G-CSF.

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Year:  1989        PMID： 2473053      PMCID： PMC5917736          DOI： 10.1111/j.1349-7006.1989.tb02316.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  23 in total

1.  Functional and morphological characterization of cultures of Kupffer cells and liver endothelial cells prepared by means of density separation in Percoll, and selective substrate adherence.

Authors:  B Smedsrød; H Pertoft; G Eggertsen; C Sundström
Journal:  Cell Tissue Res       Date:  1985       Impact factor: 5.249

2.  Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1).

Authors:  E S Kawasaki; M B Ladner; A M Wang; J Van Arsdell; M K Warren; M Y Coyne; V L Schweickart; M T Lee; K J Wilson; A Boosman
Journal:  Science       Date:  1985-10-18       Impact factor: 47.728

3.  Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells.

Authors:  L M Souza; T C Boone; J Gabrilove; P H Lai; K M Zsebo; D C Murdock; V R Chazin; J Bruszewski; H Lu; K K Chen; J Barendt; E Platzer; M A S Moore; R Mertelsmann; K Welte
Journal:  Science       Date:  1986-04-04       Impact factor: 47.728

4.  Purification and characterization of human colony-stimulating factor 1 from human pancreatic carcinoma (MIA PaCa-2) cells.

Authors:  J H Shieh; J K Cini; M C Wu; A A Yunis
Journal:  Arch Biochem Biophys       Date:  1987-02-15       Impact factor: 4.013

5.  Establishment of a CSF-producing cell line from a human gastric carcinoma and characteristics of the CSF produced by that cell line.

Authors:  M Ueda; Y Fujiwara; A Murakami; M Takahashi; H Mizoguchi
Journal:  Int J Cell Cloning       Date:  1987-07

6.  Production of granulocyte colony-stimulating factor by a human melanoma cell line.

Authors:  M B Lilly; P E Devlin; J J Devlin; T A Rado
Journal:  Exp Hematol       Date:  1987-10       Impact factor: 3.084

7.  Production of interleukin 1 alpha-like factor and colony-stimulating factor by a squamous cell carcinoma of the thyroid (T3M-5) derived from a patient with hypercalcemia and leukocytosis.

Authors:  K Sato; Y Fujii; M Ono; H Nomura; K Shizume
Journal:  Cancer Res       Date:  1987-12-15       Impact factor: 12.701

8.  Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor.

Authors:  S Nagata; M Tsuchiya; S Asano; Y Kaziro; T Yamazaki; O Yamamoto; Y Hirata; N Kubota; M Oheda; H Nomura
Journal:  Nature       Date:  1986 Jan 30-Feb 5       Impact factor: 49.962

9.  Biologic properties in vitro of a recombinant human granulocyte-macrophage colony-stimulating factor.

Authors:  D Metcalf; C G Begley; G R Johnson; N A Nicola; M A Vadas; A F Lopez; D J Williamson; G G Wong; S C Clark; E A Wang
Journal:  Blood       Date:  1986-01       Impact factor: 22.113

10.  Bipotential murine hemopoietic cell line (NFS-60) that is responsive to IL-3, GM-CSF, G-CSF, and erythropoietin.

Authors:  K Hara; T Suda; J Suda; M Eguchi; J N Ihle; S Nagata; Y Miura; M Saito
Journal:  Exp Hematol       Date:  1988-05       Impact factor: 3.084
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  3 in total

1.  Urinary bladder carcinoma producing granulocyte colony stimulating factor (G-CSF): a case report with immunohistochemistry.

Authors:  T Ito; K Shimamura; K Shoji; A Akatsuka; Y Kiryu; N Tamaoki; Y Osamura
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1993

2.  Establishment and characterization of a new lung cancer cell line (MI-4) producing high levels of granulocyte colony stimulating factor.

Authors:  Ryoji Harada; Yoshiki Uemura; Makoto Kobayashi; Adriana Zamecnikova; Hideshi Nakata; Takahiro Taguchi; Mutsuo Furihata; Yuji Otsuki; Hirokuni Taguchi
Journal:  Jpn J Cancer Res       Date:  2002-06

3.  Production of multiple growth factors by a newly established human thyroid carcinoma cell line.

Authors:  K Tohyama; Y Yoshida; K Ohashi; E Sano; H Kobayashi; K Endo; M Naruto; T Nakamura
Journal:  Jpn J Cancer Res       Date:  1992-02
  3 in total

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