Matyáš Fendrych1, Tom Van Hautegem1, Matthias Van Durme1, Yadira Olvera-Carrillo1, Marlies Huysmans1, Mansour Karimi1, Saskia Lippens2, Christopher J Guérin2, Melanie Krebs3, Karin Schumacher3, Moritz K Nowack4. 1. Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium. 2. Bio Imaging Core, VIB, 9052 Ghent, Belgium; Inflammation Research Center, VIB, 9052 Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, 9052 Ghent, Belgium. 3. Plant Developmental Biology, Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany. 4. Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium. Electronic address: moritz.nowack@vib.be.
Abstract
BACKGROUND: The root cap is a plant organ that ensheathes the meristematic stem cells at the root tip. Unlike other plant organs, the root cap shows a rapid cellular turnover, balancing constant cell generation by specific stem cells with the disposal of differentiated cells at the root cap edge. This cellular turnover is critical for the maintenance of root cap size and its position around the growing root tip, but how this is achieved and controlled in the model plant Arabidopsis thaliana remains subject to contradictory hypotheses. RESULTS: Here, we show that a highly organized cell death program is the final step of lateral root cap differentiation and that preparation for cell death is transcriptionally controlled by ANAC033/SOMBRERO. Precise timing of cell death is critical for the elimination of root cap cells before they fully enter the root elongation zone, which in turn is important in order to allow optimal root growth. Root cap cell death is followed by a rapid cell-autonomous corpse clearance and DNA fragmentation dependent on the S1-P1 type nuclease BFN1. CONCLUSIONS: Based on these results, we propose a novel concept in plant development that recognizes programmed cell death as a mechanism for maintaining organ size and tissue homeostasis in the Arabidopsis root cap.
BACKGROUND: The root cap is a plant organ that ensheathes the meristematic stem cells at the root tip. Unlike other plant organs, the root cap shows a rapid cellular turnover, balancing constant cell generation by specific stem cells with the disposal of differentiated cells at the root cap edge. This cellular turnover is critical for the maintenance of root cap size and its position around the growing root tip, but how this is achieved and controlled in the model plant Arabidopsis thaliana remains subject to contradictory hypotheses. RESULTS: Here, we show that a highly organized cell death program is the final step of lateral root cap differentiation and that preparation for cell death is transcriptionally controlled by ANAC033/SOMBRERO. Precise timing of cell death is critical for the elimination of root cap cells before they fully enter the root elongation zone, which in turn is important in order to allow optimal root growth. Root cap cell death is followed by a rapid cell-autonomous corpse clearance and DNA fragmentation dependent on the S1-P1 type nuclease BFN1. CONCLUSIONS: Based on these results, we propose a novel concept in plant development that recognizes programmed cell death as a mechanism for maintaining organ size and tissue homeostasis in the Arabidopsis root cap.
Authors: Bonnie S Watson; Mohamed F Bedair; Ewa Urbanczyk-Wochniak; David V Huhman; Dong Sik Yang; Stacy N Allen; Wensheng Li; Yuhong Tang; Lloyd W Sumner Journal: Plant Physiol Date: 2015-02-09 Impact factor: 8.340
Authors: Marlies Huysmans; Rafael Andrade Buono; Noemi Skorzinski; Marta Cubria Radio; Freya De Winter; Boris Parizot; Jan Mertens; Mansour Karimi; Matyas Fendrych; Moritz K Nowack Journal: Plant Cell Date: 2018-08-10 Impact factor: 11.277
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