PURPOSE: The leading cause of sexually transmitted bacterial infection is Chlamydia trachomatis. The aim of this study is to investigate the early events in colonization of this bacterium within the murine genital tract. PROCEDURES: An in vivo animal body imaging technology was used to track fluorophore labeled C. muridarum elementary bodies (EBs) inoculated intravaginally in C57BL/6 mice during the first 24 h of infection. RESULTS: Ascension of viable EBs was observed (1) to be localized to the lower regions of the murine genital tract within the first 24 h post challenge and (2) was dose independent during this early exposure period. Molecular detection revealed enhanced bacterial load in lower regions of the genital tract with increasing bacterial load in the upper region beginning 12 h post inoculation. CONCLUSION: This study provides additional insight into chlamydial colonization in the murine genital tract during the first 12-24 h following inoculation.
PURPOSE: The leading cause of sexually transmitted bacterial infection is Chlamydia trachomatis. The aim of this study is to investigate the early events in colonization of this bacterium within the murine genital tract. PROCEDURES: An in vivo animal body imaging technology was used to track fluorophore labeled C. muridarum elementary bodies (EBs) inoculated intravaginally in C57BL/6 mice during the first 24 h of infection. RESULTS: Ascension of viable EBs was observed (1) to be localized to the lower regions of the murine genital tract within the first 24 h post challenge and (2) was dose independent during this early exposure period. Molecular detection revealed enhanced bacterial load in lower regions of the genital tract with increasing bacterial load in the upper region beginning 12 h post inoculation. CONCLUSION: This study provides additional insight into chlamydial colonization in the murine genital tract during the first 12-24 h following inoculation.
Authors: Sangamithra Kamalakaran; Bharat K R Chaganty; Rishein Gupta; M Neal Guentzel; James P Chambers; Ashlesh K Murthy; Bernard P Arulanandam Journal: Front Cell Infect Microbiol Date: 2013-03-11 Impact factor: 5.293
Authors: J A M C Dirks; G A F S van Liere; S Bogers; N H T M Dukers-Muijrers; P F G Wolffs; C J P A Hoebe Journal: PLoS One Date: 2015-12-29 Impact factor: 3.240
Authors: Manishkumar Patel; Shu-An Lin; Melissa A Boddicker; Christopher DeMaula; Brett Connolly; Bohumil Bednar; Jon H Heinrichs; Jeffrey G Smith Journal: Mediators Inflamm Date: 2015-11-18 Impact factor: 4.711