OBJECTIVES: To validate the high-performance liquid chromatography method (HPLC) for rifampicin (RFP) determination in human plasma. MATERIALS AND METHODS: A HPLC method for RFP determination in plasma was developed. The separation was performed by reversed-phase chromatography with C18 column and a mobile phase composed of a mixture of acetonitrile and monobasic potassium phosphate buffer solution 0.05 M (38:62 v/v) at 335 nm in which standard rifampicin quinone (RFP-QN) was used. RESULTS: The retention times of RFP and RFP-QN were 7.81 and 12.26 minutes, respectively. The trial was linear from 0.5 to 250 ug/mL. The evaluated parameters of precision, accuracy, selectivity, linearity, and recovery complied with the established international standards for validation of bioanalytical methods. CONCLUSIONS: The developed HPLC method is simple, specific, sensitive, selective and linear for a wide range of RFP concentrations in plasma.
OBJECTIVES: To validate the high-performance liquid chromatography method (HPLC) for rifampicin (RFP) determination in human plasma. MATERIALS AND METHODS: A HPLC method for RFP determination in plasma was developed. The separation was performed by reversed-phase chromatography with C18 column and a mobile phase composed of a mixture of acetonitrile and monobasic potassium phosphate buffer solution 0.05 M (38:62 v/v) at 335 nm in which standard rifampicin quinone (RFP-QN) was used. RESULTS: The retention times of RFP and RFP-QN were 7.81 and 12.26 minutes, respectively. The trial was linear from 0.5 to 250 ug/mL. The evaluated parameters of precision, accuracy, selectivity, linearity, and recovery complied with the established international standards for validation of bioanalytical methods. CONCLUSIONS: The developed HPLC method is simple, specific, sensitive, selective and linear for a wide range of RFP concentrations in plasma.
Authors: Mohammad F Khan; Shamima A Rita; Md Shahidulla Kayser; Md Shariful Islam; Sharmeen Asad; Ridwan Bin Rashid; Md Abdul Bari; Muhammed M Rahman; D A Anwar Al Aman; Nurul I Setu; Rebecca Banoo; Mohammad A Rashid Journal: Front Chem Date: 2017-04-28 Impact factor: 5.221
Authors: Ana Santoveña-Estévez; Javier Suárez-González; Amor R Cáceres-Pérez; Zuleima Ruiz-Noda; Sara Machado-Rodríguez; Magdalena Echezarreta; Mabel Soriano; José B Fariña Journal: Pharmaceutics Date: 2020-02-24 Impact factor: 6.321