| Literature DB >> 24718324 |
Kazunori Masahata1, Eiji Umemoto2, Hisako Kayama2, Manato Kotani3, Shota Nakamura4, Takashi Kurakawa5, Junichi Kikuta3, Kazuyoshi Gotoh4, Daisuke Motooka4, Shintaro Sato6, Tomonori Higuchi7, Yoshihiro Baba8, Tomohiro Kurosaki8, Makoto Kinoshita2, Yosuke Shimada5, Taishi Kimura5, Ryu Okumura5, Akira Takeda5, Masaru Tajima9, Osamu Yoshie7, Masahiro Fukuzawa10, Hiroshi Kiyono11, Sidonia Fagarasan12, Tetsuya Iida13, Masaru Ishii14, Kiyoshi Takeda2.
Abstract
Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.Entities:
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Year: 2014 PMID： 24718324 DOI： 10.1038/ncomms4704
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919