Pankaj Suman1, Satish Kumar Gupta. 1. Reproductive Cell Biology Laboratory, National Institute of Immunology, New Delhi, India.
Abstract
PROBLEM: The aim of this study was to investigate the relative importance of STAT3 and ERK1/2 activation in leukaemia inhibitory factor (LIF)-mediated invasion of JEG-3 cells. METHOD OF STUDY: Matrigel matrix-based invasion assay; Western blot; cDNA microarray; quantitative RT-PCR; gene silencing by siRNA. RESULTS: Leukaemia inhibitory factor treatment led to the activation of STAT3 and ERK1/2 signaling pathways which was followed by changes in the expression of several invasion-associated molecules such as mucin1 (MUC1), Fos, Jun, etc. Abrogation of either STAT3 or ERK1/2 signaling reduced (P < 0.05) the LIF-mediated invasion of JEG-3 cells. It was associated with a significant reduction in the expression of both MUC1 and Fos, suggesting a common denominator in LIF-STAT3-ERK1/2 signaling. To this effect, we observed a decrease in LIF-mediated p-STAT3 (Ser727) upon blocking STAT3 or ERK1/2 signaling. CONCLUSIONS: ERK1/2 as well as JAK-STAT-mediated STAT3 (Ser727) phosphorylation play an important role in LIF-mediated JEG-3 trophoblastic cell invasion and gene expression.
PROBLEM: The aim of this study was to investigate the relative importance of STAT3 and ERK1/2 activation in leukaemia inhibitory factor (LIF)-mediated invasion of JEG-3 cells. METHOD OF STUDY: Matrigel matrix-based invasion assay; Western blot; cDNA microarray; quantitative RT-PCR; gene silencing by siRNA. RESULTS:Leukaemia inhibitory factor treatment led to the activation of STAT3 and ERK1/2 signaling pathways which was followed by changes in the expression of several invasion-associated molecules such as mucin1 (MUC1), Fos, Jun, etc. Abrogation of either STAT3 or ERK1/2 signaling reduced (P < 0.05) the LIF-mediated invasion of JEG-3 cells. It was associated with a significant reduction in the expression of both MUC1 and Fos, suggesting a common denominator in LIF-STAT3-ERK1/2 signaling. To this effect, we observed a decrease in LIF-mediated p-STAT3 (Ser727) upon blocking STAT3 or ERK1/2 signaling. CONCLUSIONS:ERK1/2 as well as JAK-STAT-mediated STAT3 (Ser727) phosphorylation play an important role in LIF-mediated JEG-3 trophoblastic cell invasion and gene expression.
Authors: Shavahn C Loux; Kirsten E Scoggin; Jason E Bruemmer; Igor F Canisso; Mats H T Troedsson; Edward L Squires; Barry A Ball Journal: PLoS One Date: 2017-04-07 Impact factor: 3.240