PURPOSE: Archived formalin-fixed paraffin-embedded (FFPE) tissue collections represent a valuable informational resource for numerous studies. However, there is no NMR signal from FFPE specimens at room temperature. To obtain MR images and enable comparison of magnetic resonance microscopy (MRM) and histology studies we propose to image FFPE tissue at elevated temperature. METHODS: A FFPE tissue block was imaged at 7 Tesla (T) and 16.4T at 70-80°C using T2 -weighting methods. The only difference from standard MR microscopy (MRM) is elevated temperature at which the embedding medium melts. RESULTS: Using FFPE murine brain tissue, we were able to demonstrate the feasibility of tissue MRM from paraffin embedded specimens. Histology images taken from the specimen after MRM demonstrate that keeping the FFPE specimen in paraffin melt at 70-80°C for dozens of hours does not affect subsequent histology analysis. CONCLUSION: MRM of FFPE tissue from paraffin melt opens new avenues for analyzing archived histological specimens (biopsies, post mortem, etc.) and for correlating MR images with histology (optical microscopy). In addition, 3D MRM of FFPE specimens could guide histology in search for appropriate regions of interest and subsequently minimize occurrences of false negatives in tissue analysis.
PURPOSE: Archived formalin-fixed paraffin-embedded (FFPE) tissue collections represent a valuable informational resource for numerous studies. However, there is no NMR signal from FFPE specimens at room temperature. To obtain MR images and enable comparison of magnetic resonance microscopy (MRM) and histology studies we propose to image FFPE tissue at elevated temperature. METHODS: A FFPE tissue block was imaged at 7 Tesla (T) and 16.4T at 70-80°C using T2 -weighting methods. The only difference from standard MR microscopy (MRM) is elevated temperature at which the embedding medium melts. RESULTS: Using FFPE murine brain tissue, we were able to demonstrate the feasibility of tissue MRM from paraffin embedded specimens. Histology images taken from the specimen after MRM demonstrate that keeping the FFPE specimen in paraffin melt at 70-80°C for dozens of hours does not affect subsequent histology analysis. CONCLUSION: MRM of FFPE tissue from paraffin melt opens new avenues for analyzing archived histological specimens (biopsies, post mortem, etc.) and for correlating MR images with histology (optical microscopy). In addition, 3D MRM of FFPE specimens could guide histology in search for appropriate regions of interest and subsequently minimize occurrences of false negatives in tissue analysis.
Authors: Alice Ly; Achim Buck; Benjamin Balluff; Na Sun; Karin Gorzolka; Annette Feuchtinger; Klaus-Peter Janssen; Peter J K Kuppen; Cornelis J H van de Velde; Gregor Weirich; Franziska Erlmeier; Rupert Langer; Michaela Aubele; Horst Zitzelsberger; Liam McDonnell; Michaela Aichler; Axel Walch Journal: Nat Protoc Date: 2016-07-14 Impact factor: 13.491
Authors: Inga Langner; Thomas Stahnke; Oliver Stachs; Tobias Lindner; Jens-Peter Kühn; Simon Kim; Andreas Wree; Soenke Langner Journal: BMC Dev Biol Date: 2016-06-18 Impact factor: 1.978
Authors: Wing Ying Chow; Brendan P Norman; Norman B Roberts; Lakshminarayan R Ranganath; Christian Teutloff; Robert Bittl; Melinda J Duer; James A Gallagher; Hartmut Oschkinat Journal: Angew Chem Int Ed Engl Date: 2020-05-14 Impact factor: 15.336