Literature DB >> 2471515

Kinetic evidence for a two-step mechanism for the binding of chymotrypsin to alpha 1-proteinase inhibitor.

M Bruch1, J G Bieth.   

Abstract

We have used the proflavin displacement method and a stopped-flow apparatus to measure the rate constant for the binding of 2 microM-chymotrypsin to 20-125 microM-alpha 1-proteinase inhibitor. The observed pseudo-first-order constant showed a hyperbolic dependence on alpha 1-proteinase inhibitor concentration, suggesting a reaction mechanism in which a fast pre-equilibrium (K = 0.19 mM) is followed by a first-order formation of the final complex (k = 252 s-1).

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Year:  1989        PMID： 2471515      PMCID： PMC1138609          DOI： 10.1042/bj2590929

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  The spectrophotometric determination of the operational normality of an alpha-chymotrypsin solution.

Authors:  G R SCHONBAUM; B ZERNER; M L BENDER
Journal:  J Biol Chem       Date:  1961-11       Impact factor: 5.157

2.  Plasma serine proteinase inhibitors (serpins) exhibit major conformational changes and a large increase in conformational stability upon cleavage at their reactive sites.

Authors:  M Bruch; V Weiss; J Engel
Journal:  J Biol Chem       Date:  1988-11-15       Impact factor: 5.157

3.  Kinetics of the interaction of bovine pancreatic trypsin inhibitor (Kunitz) with alpha-chymotrypsin.

Authors:  U Quast; J Engel; H Heumann; G Krause; E Steffen
Journal:  Biochemistry       Date:  1974-06-04       Impact factor: 3.162

4.  Spectral studies of the interaction of alpha-chymotrypsin and trypsin with proflavine.

Authors:  A N Glazer
Journal:  Proc Natl Acad Sci U S A       Date:  1965-07       Impact factor: 11.205

Review 5.  Human plasma proteinase inhibitors.

Authors:  J Travis; G S Salvesen
Journal:  Annu Rev Biochem       Date:  1983       Impact factor: 23.643

6.  Kinetics of association of serine proteinases with native and oxidized alpha-1-proteinase inhibitor and alpha-1-antichymotrypsin.

Authors:  K Beatty; J Bieth; J Travis
Journal:  J Biol Chem       Date:  1980-05-10       Impact factor: 5.157

7.  Influence of elastin on the inhibition of leucocyte elastase by alpha 1-proteinase inhibitor and bronchial inhibitor. Potent inhibition of elastin-bound elastase by bronchial inhibitor.

Authors:  M Bruch; J G Bieth
Journal:  Biochem J       Date:  1986-08-15       Impact factor: 3.857

8.  Human alpha 1-proteinase inhibitor. Crystal structure analysis of two crystal modifications, molecular model and preliminary analysis of the implications for function.

Authors:  H Loebermann; R Tokuoka; J Deisenhofer; R Huber
Journal:  J Mol Biol       Date:  1984-08-15       Impact factor: 5.469
  8 in total
  3 in total

1.  Kinetics of the interaction of chymotrypsin with eglin c.

Authors:  B Faller; J G Bieth
Journal:  Biochem J       Date:  1991-11-15       Impact factor: 3.857

2.  Kinetics of the inhibition of human pancreatic elastase by recombinant eglin c. Influence of elastin.

Authors:  B Faller; S Dirrig; M Rabaud; J G Bieth
Journal:  Biochem J       Date:  1990-09-15       Impact factor: 3.857

3.  Structural differences between active forms of plasminogen activator inhibitor type 1 revealed by conformationally sensitive ligands.

Authors:  Shih-Hon Li; Natalia V Gorlatova; Daniel A Lawrence; Bradford S Schwartz
Journal:  J Biol Chem       Date:  2008-04-24       Impact factor: 5.157
  3 in total

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