BACKGROUND/AIMS: TFF2 is a secretory peptide (106 amino acid residues) of the gastric mucosa, the porcine exocrine pancreas as well as immune cells and the CNS. It was the aim of this study to compare gastric and pancreatic TFF2. METHODS: TFF2 was purified from the porcine stomach and pancreas, respectively, by size-exclusion and anion-exchange chromatography and then analyzed by Western blots as well as mass spectrometry. RESULTS: Gastric and pancreatic TFF2 differed markedly, i.e. gastric TFF2 was exclusively associated with the high molecular mass mucus fraction, whereas pancreatic TFF2 appeared as a low molecular mass product. Unexpectedly, the latter predominantly formed a non-covalently linked homodimer resistant to boiling SDS. In contrast, gastric TFF2 is an integral mucus constituent predominantly binding to the mucin MUC6 in a non-covalent fashion. CONCLUSION: The non-covalent interaction of TFF2 with the mucin MUC6 is typical of a "link peptide" which is perfectly suited to assemble and stabilize the laminated structure of gastric mucus and to modulate its rheological properties.
BACKGROUND/AIMS: TFF2 is a secretory peptide (106 amino acid residues) of the gastric mucosa, the porcine exocrine pancreas as well as immune cells and the CNS. It was the aim of this study to compare gastric and pancreatic TFF2. METHODS:TFF2 was purified from the porcine stomach and pancreas, respectively, by size-exclusion and anion-exchange chromatography and then analyzed by Western blots as well as mass spectrometry. RESULTS: Gastric and pancreatic TFF2 differed markedly, i.e. gastric TFF2 was exclusively associated with the high molecular mass mucus fraction, whereas pancreatic TFF2 appeared as a low molecular mass product. Unexpectedly, the latter predominantly formed a non-covalently linked homodimer resistant to boiling SDS. In contrast, gastric TFF2 is an integral mucus constituent predominantly binding to the mucin MUC6 in a non-covalent fashion. CONCLUSION: The non-covalent interaction of TFF2 with the mucin MUC6 is typical of a "link peptide" which is perfectly suited to assemble and stabilize the laminated structure of gastric mucus and to modulate its rheological properties.
Authors: Shaopeng Yuan; Martin Hollinger; Marrah E Lachowicz-Scroggins; Sheena C Kerr; Eleanor M Dunican; Brian M Daniel; Sudakshina Ghosh; Serpel C Erzurum; Belinda Willard; Stanley L Hazen; Xiaozhu Huang; Stephen D Carrington; Stefan Oscarson; John V Fahy Journal: Sci Transl Med Date: 2015-02-25 Impact factor: 17.956
Authors: Heather L Rossi; Jorge F Ortiz-Carpena; Devon Tucker; Andrew E Vaughan; Nilam S Mangalmurti; Noam A Cohen; De'Broski R Herbert Journal: Am J Respir Cell Mol Biol Date: 2022-03 Impact factor: 6.914