Alicja Chrzanowska1, Wojciech Graboń1, Magdalena Mielczarek-Puta1, Anna Barańczyk-Kuźma2. 1. Chair and Department of Biochemistry, Medical University of Warsaw, 02-097 Warsaw, Banacha 1, Poland. 2. Chair and Department of Biochemistry, Medical University of Warsaw, 02-097 Warsaw, Banacha 1, Poland. Electronic address: akuzma@wum.edu.pl.
Abstract
OBJECTIVE: To assess the utility of arginase activity and expression in diagnosis of liver diseases. DESIGN AND METHODS: Arginase activity, sensitivity and specificity were determined in serum of 140 patients including 50 with HCC, 60 with LC, 30 with choledocholithiasis (CDL) and 90 healthy controls. In HCC and LC arginase activity in serum was studied before and after tumor resection or liver transplantation. Arginase sensitivity in HCC was compared to that of alpha-fetoprotein (AFP) and aminotransferases (AST, ALT). In LC the activity was determined also in bile before and after transplantation. The expression of arginase isoenzymes in serum was studied by Western blotting. RESULTS: In HCC and LC the preoperative arginase activity was significantly higher compared to controls, and it decreased after surgery. The sensitivity of arginase in HCC was much higher than that of AFP, AST and ALT (96, 40, 20 and 18%, respectively). In HCC it was higher than in LC (93%) and CDL (33%). The specificity of arginase was above 80%. In bile of cirrhotic patients the highest activity was immediately after liver transplantation. It decreased with time but increased dramatically at the time of the graft rejection. Arginase AII was present in serum of HCC and LC but not the control cases. CONCLUSIONS: The increase of arginase activity in serum accompanied by the presence of isoenzyme AII can be useful in HCC and LC diagnosis. The determination of arginase activity in bile may be helpful in monitoring liver graft recipients.
OBJECTIVE: To assess the utility of arginase activity and expression in diagnosis of liver diseases. DESIGN AND METHODS: Arginase activity, sensitivity and specificity were determined in serum of 140 patients including 50 with HCC, 60 with LC, 30 with choledocholithiasis (CDL) and 90 healthy controls. In HCC and LC arginase activity in serum was studied before and after tumor resection or liver transplantation. Arginase sensitivity in HCC was compared to that of alpha-fetoprotein (AFP) and aminotransferases (AST, ALT). In LC the activity was determined also in bile before and after transplantation. The expression of arginase isoenzymes in serum was studied by Western blotting. RESULTS: In HCC and LC the preoperative arginase activity was significantly higher compared to controls, and it decreased after surgery. The sensitivity of arginase in HCC was much higher than that of AFP, AST and ALT (96, 40, 20 and 18%, respectively). In HCC it was higher than in LC (93%) and CDL (33%). The specificity of arginase was above 80%. In bile of cirrhotic patients the highest activity was immediately after liver transplantation. It decreased with time but increased dramatically at the time of the graft rejection. Arginase AII was present in serum of HCC and LC but not the control cases. CONCLUSIONS: The increase of arginase activity in serum accompanied by the presence of isoenzyme AII can be useful in HCC and LC diagnosis. The determination of arginase activity in bile may be helpful in monitoring liver graft recipients.
Authors: Marcin Mikołaj Grzybowski; Paulina Seweryna Stańczak; Paulina Pomper; Roman Błaszczyk; Bartłomiej Borek; Anna Gzik; Julita Nowicka; Karol Jędrzejczak; Joanna Brzezińska; Tomasz Rejczak; Nazan Cemre Güner-Chalimoniuk; Agnieszka Kikulska; Michał Mlącki; Jolanta Pęczkowicz-Szyszka; Jacek Olczak; Adam Gołębiowski; Karolina Dzwonek; Paweł Dobrzański; Zbigniew Zasłona Journal: Cancers (Basel) Date: 2022-08-17 Impact factor: 6.575