Thaisa Cezária Triches1, Luciene Cristina de Figueiredo2, Magda Feres3, Sérgio Fernando Torres de Freitas4, Gláucia Santos Zimmermann5, Mabel Mariela Rodríguez Cordeiro6. 1. Private practice, Florianópolis, Santa Catarina, Brazil. ttriches@gmail.com. 2. Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil. 3. Graduate Program in Dentistry, Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil. 4. Department of Public Health, at the School of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil. 5. Department of Periodontology, at the School of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil. 6. Department of Morphological Sciences, at the School of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Abstract
PURPOSE: The purpose of this study was to assess the microbial content of root canals of human primary teeth with pulp necrosis and periradicular lesion. METHODS: Microbial samples were collected from 24 canals of children treated at a pediatric dentistry clinic. Microbiological identification was performed using checker-board DNA-DNA hybridization for 40 different bacteria. Data were analyzed per canal based on the mean count and frequency of each bacterial species. RESULTS: Detectable levels of bacterial species were observed for 35 probes (88%). The most frequent bacteria were Fusobacterium nucleatum sp. nucleatum, Fusobacterium periodonticum, Prevotella melaninogenica, Prevotella nigrescens, and Prevotella intermedia. Facultative species were identified in 20 root canals (83%), anaerobic species were identified in 24 root canals (100%), and aerobic species in 18 root canals (75%). Black-pigmented bacilli were found in 23 samples (96%). The number of different bacterial species detected per canal ranged from five to 33. CONCLUSION: Endodontic infection in primary teeth with pulp necrosis and periradicular lesion is multimicrobial, including aerobic, facultative, and anaerobic micro-organisms.
PURPOSE: The purpose of this study was to assess the microbial content of root canals of human primary teeth with pulp necrosis and periradicular lesion. METHODS: Microbial samples were collected from 24 canals of children treated at a pediatric dentistry clinic. Microbiological identification was performed using checker-board DNA-DNA hybridization for 40 different bacteria. Data were analyzed per canal based on the mean count and frequency of each bacterial species. RESULTS: Detectable levels of bacterial species were observed for 35 probes (88%). The most frequent bacteria were Fusobacterium nucleatum sp. nucleatum, Fusobacterium periodonticum, Prevotella melaninogenica, Prevotella nigrescens, and Prevotella intermedia. Facultative species were identified in 20 root canals (83%), anaerobic species were identified in 24 root canals (100%), and aerobic species in 18 root canals (75%). Black-pigmented bacilli were found in 23 samples (96%). The number of different bacterial species detected per canal ranged from five to 33. CONCLUSION: Endodontic infection in primary teeth with pulp necrosis and periradicular lesion is multimicrobial, including aerobic, facultative, and anaerobic micro-organisms.
Authors: Natalia I Chalmers; Kevin Oh; Christopher V Hughes; Nooruddin Pradhan; Eleni Kanasi; Ygal Ehrlich; Floyd E Dewhirst; Anne C R Tanner Journal: J Oral Microbiol Date: 2015-02-03 Impact factor: 5.474
Authors: Eimear Hurley; Maurice P J Barrett; Martin Kinirons; Helen Whelton; C Anthony Ryan; Catherine Stanton; Hugh M B Harris; Paul W O'Toole Journal: BMC Oral Health Date: 2019-01-14 Impact factor: 2.757
Authors: Mary Rodríguez-Rabassa; Pablo López; Raphael Sánchez; Cyanela Hernández; Cesarly Rodríguez; Ronald E Rodríguez-Santiago; Juan C Orengo; Vivian Green; Yasuhiro Yamamura; Vanessa Rivera-Amill Journal: Int J Environ Res Public Health Date: 2020-01-21 Impact factor: 3.390