Raffaele Serra1, Raffaele Grande2, Luca Gallelli3, Pierandrea Rende3, Edoardo Scarcello4, Gianluca Buffone2, Francesco Giuseppe Caliò5, Vincenzo Gasbarro6, Bruno Amato7, Stefano de Franciscis2. 1. Department of Medical and Surgical Science, University Magna Graecia of Catanzaro, Catanzaro, Italy. Electronic address: rserra@unicz.it. 2. Department of Medical and Surgical Science, University Magna Graecia of Catanzaro, Catanzaro, Italy. 3. Department of Health Science, University Magna Graecia of Catanzaro, Catanzaro, Italy. 4. Unit of Vascular Surgery, Annunziata Hospital, Cosenza, Italy. 5. Unit of Vascular Surgery, S. Anna Hospital, Catanzaro, Italy. 6. Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy. 7. Department of General, Geriatric, Oncologic Surgery and Advanced Technologies, University of Naples "Federico II", Naples, Italy.
Abstract
BACKGROUND: The carotid body tumors (CBTs) are relatively rare neoplasms and may present into benign or life threatening malignant forms. Matrix metalloproteinases (MMPs) are often involved in vascular and cancer diseases. Objective of this study is to study the relationship between CBTs and MMPs. METHODS: We performed a multicenter study on 14 patients with CBTs. All tumors were resected. For each patient, we evaluated the MMPs' levels in both plasma (enzyme-linked immunosorbent assay [ELISA] test) and tissue samples (Western blot analysis). These MMPs' plasma levels were compared with the MMPs' plasma levels of healthy patients. RESULTS: Eleven patients had benign CBTs, whereas 3 patients had malignant CBTs. ELISA findings revealed significant higher levels (P < 0.01) of MMP-1, -2, -3, -8, and -9 in patients with paraganglioma with respect to healthy patients. Patients with malignant CBTs showed significantly higher levels (P < 0.01) of MMP-1, -2, and -3 compared with patients with benign CBTs. CONCLUSIONS: Because this is an exploratory study, the experience on this casuistry showed that MMPs' evaluation may help clinicians and surgeons to formulate a more rapid and clear diagnosis on CBTs' behavior. However, other studies on a large group of patients may be useful to validate these observations.
BACKGROUND: The carotid body tumors (CBTs) are relatively rare neoplasms and may present into benign or life threatening malignant forms. Matrix metalloproteinases (MMPs) are often involved in vascular and cancer diseases. Objective of this study is to study the relationship between CBTs and MMPs. METHODS: We performed a multicenter study on 14 patients with CBTs. All tumors were resected. For each patient, we evaluated the MMPs' levels in both plasma (enzyme-linked immunosorbent assay [ELISA] test) and tissue samples (Western blot analysis). These MMPs' plasma levels were compared with the MMPs' plasma levels of healthy patients. RESULTS: Eleven patients had benign CBTs, whereas 3 patients had malignant CBTs. ELISA findings revealed significant higher levels (P < 0.01) of MMP-1, -2, -3, -8, and -9 in patients with paraganglioma with respect to healthy patients. Patients with malignant CBTs showed significantly higher levels (P < 0.01) of MMP-1, -2, and -3 compared with patients with benign CBTs. CONCLUSIONS: Because this is an exploratory study, the experience on this casuistry showed that MMPs' evaluation may help clinicians and surgeons to formulate a more rapid and clear diagnosis on CBTs' behavior. However, other studies on a large group of patients may be useful to validate these observations.
Authors: Stefano de Franciscis; Giovanni De Caridi; Mafalda Massara; Francesco Spinelli; Luca Gallelli; Gianluca Buffone; Francesco G Caliò; Lucia Butrico; Raffaele Grande; Raffaele Serra Journal: Int Wound J Date: 2014-12-03 Impact factor: 3.315
Authors: Giovanni De Caridi; Mafalda Massara; Francesco Stilo; Francesco Spinelli; Raffaele Grande; Lucia Butrico; Stefano de Franciscis; Raffaele Serra Journal: Int Wound J Date: 2014-08-05 Impact factor: 3.315
Authors: Diego José Rojas Esquivel; Stefan Stefanov Kiuri; Alvaro Fernández Heredero; Luis Riera De Cubas; José Cebrian Carretero Journal: SAGE Open Med Case Rep Date: 2014-11-19
Authors: Giovanni De Caridi; Mafalda Massara; Francesco Spinelli; Raffaele Grande; Lucia Butrico; Pierandrea Rende; Maurizio Amato; Rita Compagna; Bruno Amato; Stefano de Franciscis; Raffaele Serra Journal: Open Med (Wars) Date: 2015-12-17
Authors: Raffaele Serra; Luca Gallelli; Angela Conti; Giovanni De Caridi; Mafalda Massara; Francesco Spinelli; Gianluca Buffone; Francesco Giuseppe Caliò; Bruno Amato; Simona Ceglia; Giuseppe Spaziano; Luca Scaramuzzino; Alessia Giovanna Ferrarese; Raffaele Grande; Stefano de Franciscis Journal: Drug Des Devel Ther Date: 2014-05-13 Impact factor: 4.162
Authors: Aldo Rocca; Domenico Tafuri; Marianna Paccone; Antonio Giuliani; Anna Ginevra Immacolata Zamboli; Giuseppe Surfaro; Andrea Paccone; Rita Compagna; Maurizo Amato; Raffaele Serra; Bruno Amato Journal: Open Med (Wars) Date: 2017-10-21