Chunfang Qiu1, Daniel A Enquobahrie2, Ihunnaya O Frederick3, Tanya K Sorensen3, Miguel Angel Luque Fernandez4, Robert M David5, J Alexander Bralley5, Michelle A Williams6. 1. Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA. Electronic address: Chun-fang.Qiu@Swedish.org. 2. Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA; Department of Epidemiology, School of Public Health, University of Washington, Seattle, WA, USA. 3. Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA. 4. Department of Epidemiology, Harvard School of Public Health, Boston, MA, USA. 5. Genova Diagnostics, Duluth, GA, USA. 6. Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA; Department of Epidemiology, Harvard School of Public Health, Boston, MA, USA.
Abstract
AIMS: Alterations in organic acid biomarkers from fatty acid and carbohydrate metabolism have been documented in type 2 diabetes patients. However, their association with gestational diabetes mellitus (GDM) is largely unknown. METHODS: Participants were 25 GDM cases and 25 non-GDM controls. Biomarkers of fatty acid (adipate, suberate and ethylmalonate) and carbohydrate (pyruvate, l-lactate and β-hydroxybutyrate) metabolism were measured in maternal urine samples collected in early pregnancy (17 weeks) using liquid chromatography-mass spectrometry methods. Logistic regression were used to calculate odds ratios (OR) and 95% confidence intervals (CI). RESULTS: GDM cases and controls differed in median urinary concentrations of ethylmalonate (3.0 vs. 2.3μg/mg creatinine), pyruvate (7.4 vs. 2.1μg/mg creatinine), and adipate (4.6 vs. 7.3μg/mg creatinine) (all p-values <0.05). Women in the highest tertile for ethylmalonate or pyruvate concentrations had 11.4-fold (95%CI 1.10-117.48) and 3.27-fold (95%CI 0.72-14.79) increased risk of GDM compared with women in the lowest tertile for ethylmalonate and pyruvate concentrations, respectively. Women in the highest tertile for adipate concentrations, compared with women in the lowest tertile, had an 86% reduction in GDM risk (95%CI 0.02-0.97). CONCLUSIONS: These preliminary findings underscore the importance of altered fatty acid and carbohydrate metabolism in the pathogenesis of GDM.
AIMS: Alterations in organic acid biomarkers from fatty acid and carbohydrate metabolism have been documented in type 2 diabetespatients. However, their association with gestational diabetes mellitus (GDM) is largely unknown. METHODS:Participants were 25 GDM cases and 25 non-GDM controls. Biomarkers of fatty acid (adipate, suberate and ethylmalonate) and carbohydrate (pyruvate, l-lactate and β-hydroxybutyrate) metabolism were measured in maternal urine samples collected in early pregnancy (17 weeks) using liquid chromatography-mass spectrometry methods. Logistic regression were used to calculate odds ratios (OR) and 95% confidence intervals (CI). RESULTS: GDM cases and controls differed in median urinary concentrations of ethylmalonate (3.0 vs. 2.3μg/mg creatinine), pyruvate (7.4 vs. 2.1μg/mg creatinine), and adipate (4.6 vs. 7.3μg/mg creatinine) (all p-values <0.05). Women in the highest tertile for ethylmalonate or pyruvate concentrations had 11.4-fold (95%CI 1.10-117.48) and 3.27-fold (95%CI 0.72-14.79) increased risk of GDM compared with women in the lowest tertile for ethylmalonate and pyruvate concentrations, respectively. Women in the highest tertile for adipate concentrations, compared with women in the lowest tertile, had an 86% reduction in GDM risk (95%CI 0.02-0.97). CONCLUSIONS: These preliminary findings underscore the importance of alteredfatty acid and carbohydrate metabolism in the pathogenesis of GDM.
Authors: Daniel A Enquobahrie; Amy Moore; Seid Muhie; Mahlet G Tadesse; Shili Lin; Michelle A Williams Journal: Reprod Sci Date: 2015-02-11 Impact factor: 3.060
Authors: B Gelaye; C B Clish; M Denis; G Larrabure; M G Tadesse; A Deik; K Pierce; K Bullock; C Dennis; D A Enquobahrie; M A Williams Journal: Diabetes Metab Date: 2018-01-11 Impact factor: 6.041
Authors: Bizu Gelaye; Susan J Sumner; Susan McRitchie; James E Carlson; Cande V Ananth; Daniel A Enquobahrie; Chunfang Qiu; Tanya K Sorensen; Michelle A Williams Journal: PLoS One Date: 2016-06-14 Impact factor: 3.240