Literature DB >> 2470336

High titre Hog Cholera virus production on Cytodex 3 microcarrier cultures.

A Caij1, A De Smet, N Dubois, F Koenen.   

Abstract

In an attempt to produce Hog Cholera virus (HCV) preparations of high titre, optimal growth and trypsinization conditions of PK-15 microcarrier cell cultures were defined. Infecting a PK-15 Cytodex 3 microcarrier culture with HCV increased the yield of virus more than 10 times compared with conventional monolayer culture in Roux flasks.

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Year:  1989        PMID： 2470336     DOI： 10.1007/bf01311121

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  14 in total

1.  STANDARDIZATION OF HOG CHOLERA NEUTRALIZATION TEST.

Authors:  L COGGINS; J A BAKER
Journal:  Am J Vet Res       Date:  1964-03       Impact factor: 1.156

2.  The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of DEAE sephadex A50 beads.

Authors:  R E Spier; J P Whiteside
Journal:  Biotechnol Bioeng       Date:  1976-05       Impact factor: 4.530

3.  Subpassaging cells on microcarriers: the importance for scaling up to production.

Authors:  E Lindner; A C Arvidsson; I Wergeland; D Billig
Journal:  Dev Biol Stand       Date:  1987

4.  Production of Aujeszky vaccine by the microcarrier technology "from the ampoule to the 500 litre fermentor".

Authors:  B Baijot; M Duchene; J Stephenne
Journal:  Dev Biol Stand       Date:  1987

5.  Production of an inactivated rabies vaccine in primary dog kidney cells.

Authors:  A L van Wezel; G van Steenis
Journal:  Dev Biol Stand       Date:  1978

6.  Studies on the substructure of togaviruses. II. Analysis of equine arteritis, rubella, bovine viral diarrhea, and hog cholera viruses.

Authors:  M Horzinek; J Maess; R Laufs
Journal:  Arch Gesamte Virusforsch       Date:  1971

7.  Purification and some physical properties of hog cholera virus.

Authors:  C Ushimi; M Tajima; S Tanaka; H Nakajima; Y Shimizu
Journal:  Natl Inst Anim Health Q (Tokyo)       Date:  1969

8.  The large-scale cultivation of VERO cells in micro-carrier culture for virus vaccine production. Preliminary results for killed poliovirus vaccine.

Authors:  B J Montagnon; B Fanget; A J Nicolas
Journal:  Dev Biol Stand       Date:  1981

9.  Porcine parvovirus: propagation in microcarrier cell culture and immunogenic evaluation in pregnant gilts.

Authors:  E Rivera; C G Sjösten; R Bergman; K A Karlsson
Journal:  Res Vet Sci       Date:  1986-11       Impact factor: 2.534

10.  Microcarriers as a culturing system of insect cells and insect viruses.

Authors:  A Lazar; L Silberstein; S Reuveny; A Mizrahi
Journal:  Dev Biol Stand       Date:  1987
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  3 in total

1.  Characterization of structural and non-structural proteins of hog cholera virus by means of monoclonal antibodies.

Authors:  G Muyldermans; A Caij; A De Smet; F Koenen; R Hamers
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

2.  Production and characterization of monoclonal antibodies against hog cholera virus (Alfort 187 strain).

Authors:  A Caij; G Muyldermans; A De Smet; R Hamers; F Koenen
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

3.  Polymerase chain reaction-mediated cloning and in vitro translation of the genes coding for the structural proteins of hog cholera virus.

Authors:  G Muyldermans; M C San Gabriel; A Caij; A De Smet; R Hamers
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574
  3 in total

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