Vamberto O A Maia-Filho1, Andre M Rocha2, Fernando P Ferreira3, Tatiana C S Bonetti4, Paulo Serafini5, Eduardo L A Motta6. 1. Gynecology Department of Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Sao Paulo, Brazil Centre of Human Reproduction, Hospital e Maternidade Santa Joana, São Paulo, Brazil vambertof@gmail.com. 2. Huntington Reproductive Medicine, Av República do Líbano, São Paulo, Brazil. 3. Gynecology Department of Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Sao Paulo, Brazil Centre of Human Reproduction, Hospital e Maternidade Santa Joana, São Paulo, Brazil. 4. Gynecology Department of Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Sao Paulo, Brazil. 5. Centre of Human Reproduction, Hospital e Maternidade Santa Joana, São Paulo, Brazil Huntington Reproductive Medicine, Av República do Líbano, São Paulo, Brazil Gynecology Discipline of School of Medicine, University of Sao Paulo (USP), Sao Paulo, Brazil. 6. Gynecology Department of Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Sao Paulo, Brazil Centre of Human Reproduction, Hospital e Maternidade Santa Joana, São Paulo, Brazil Huntington Reproductive Medicine, Av República do Líbano, São Paulo, Brazil.
Abstract
OBJECTIVE: To evaluate the expression of endometrial matrix metalloproteinases (MMPs) 2 and 9 and E-cadherin in peri-implantation phase of infertile women who have undergone in vitro fertilization (IVF) cycles. METHODS: This prospective study included 51 patients who underwent endometrial biopsy during the receptive phase in a menstrual cycle prior to IVF treatment. The samples were evaluated by tissue microarray for immunohistochemical study. RESULTS: The expression of MMP-2, MMP-9, and E-cadherin in the endometrium prior to IVF treatment was not associated with pregnancy. There was a decrease in E-cadherin immunodetection, the higher the age of the patients, a negative relationship between E-cadherin and MMP-2, and a positive association between MMP-9 and E-cadherin. CONCLUSIONS: The MMP-2, MMP-9, and E-cadherin are expressed in the endometrium of infertile patients during the receptive phase of the natural menstrual cycle. However, there is no correlation between the expression of these molecules and the clinical IVF outcomes.
OBJECTIVE: To evaluate the expression of endometrial matrix metalloproteinases (MMPs) 2 and 9 and E-cadherin in peri-implantation phase of infertile women who have undergone in vitro fertilization (IVF) cycles. METHODS: This prospective study included 51 patients who underwent endometrial biopsy during the receptive phase in a menstrual cycle prior to IVF treatment. The samples were evaluated by tissue microarray for immunohistochemical study. RESULTS: The expression of MMP-2, MMP-9, and E-cadherin in the endometrium prior to IVF treatment was not associated with pregnancy. There was a decrease in E-cadherin immunodetection, the higher the age of the patients, a negative relationship between E-cadherin and MMP-2, and a positive association between MMP-9 and E-cadherin. CONCLUSIONS: The MMP-2, MMP-9, and E-cadherin are expressed in the endometrium of infertile patients during the receptive phase of the natural menstrual cycle. However, there is no correlation between the expression of these molecules and the clinical IVF outcomes.
Authors: V Noë; B Fingleton; K Jacobs; H C Crawford; S Vermeulen; W Steelant; E Bruyneel; L M Matrisian; M Mareel Journal: J Cell Sci Date: 2001-01 Impact factor: 5.285
Authors: Ramóna Pap; Gergely Montskó; Gergely Jánosa; Katalin Sipos; Gábor L Kovács; Edina Pandur Journal: Int J Mol Sci Date: 2020-04-30 Impact factor: 5.923