Li Bing1, Chen Hong, Shang Li-Xin, Gao Wei. 1. Department of Gynecology and Obstetrics, The Military General Hospital of Beijing PLA, Beijing, 100700, China.
Abstract
INTRODUCTION: Focal adhesion kinase (FAK) is a critical mediator of extracellular matrix signaling, cell survival, proliferation and motility. Twist homolog 1 (TWIST1) is a transcription factor and serves as a powerful oncogene. Increased FAK and TWIST1 expression are observed in a variety of solid human tumors and correlate with metastasis and poor survival. MATERIALS AND METHODS: Here, we identify miR-543 as a direct regulator of FAK and TWIST1 expression in endometrial cancer. RESULTS: Forced expression of miR-543 in endometrial cancer cell lines decreases both endogenous FAK and TWIST1 mRNA and protein levels. Forced expression of miR-543 in aggressive endometrial cancer cell lines also impairs tumor cell monolayer proliferation, anchorage-independent growth, migration and invasion. Endogenous miR-543 expression is decreased in malignant versus normal endometrium tissue and the levels of miR-543 inversely correlate with mRNA levels of FAK and TWIST1. CONCLUSIONS: miR-543 expression is decreased in endometrial cancer and serves as a tumor suppressor by targeting FAK and TWIST1 expression.
INTRODUCTION:Focal adhesion kinase (FAK) is a critical mediator of extracellular matrix signaling, cell survival, proliferation and motility. Twist homolog 1 (TWIST1) is a transcription factor and serves as a powerful oncogene. Increased FAK and TWIST1 expression are observed in a variety of solid humantumors and correlate with metastasis and poor survival. MATERIALS AND METHODS: Here, we identify miR-543 as a direct regulator of FAK and TWIST1 expression in endometrial cancer. RESULTS: Forced expression of miR-543 in endometrial cancer cell lines decreases both endogenous FAK and TWIST1 mRNA and protein levels. Forced expression of miR-543 in aggressive endometrial cancer cell lines also impairs tumor cell monolayer proliferation, anchorage-independent growth, migration and invasion. Endogenous miR-543 expression is decreased in malignant versus normal endometrium tissue and the levels of miR-543 inversely correlate with mRNA levels of FAK and TWIST1. CONCLUSIONS:miR-543 expression is decreased in endometrial cancer and serves as a tumor suppressor by targeting FAK and TWIST1 expression.
Authors: Lei Yu; Luting Zhou; Yu Cheng; Lei Sun; Jian Fan; Jinlong Liang; Mujie Guo; Ning Liu; Liying Zhu Journal: Am J Cancer Res Date: 2014-11-19 Impact factor: 6.166
Authors: Ching-Chia Wang; Lihua Ying; Elizabeth A Barnes; Eloa S Adams; Francis Y Kim; Karl W Engel; Cristina M Alvira; David N Cornfield Journal: Am J Physiol Lung Cell Mol Physiol Date: 2018-05-10 Impact factor: 5.464