Evidence for the importance of O antigen specific antibodies in mouse-protective Salmonella outer membrane protein (porin) antisera.

The antibodies responsible for the protective effect of sera obtained by immunizing rabbits with the major outer membrane protein porin of Salmonella typhimurium complexed with LPS (Kuusi et al., Infect. Immun., 1981; 34:328-332) were found to be directed to the O antigen. They were effective in very small concentration which probably accounted for our failure to identify them before or now by in vitro methods. Although both the porin and the LPS in the complexes used for immunization were isolated from rough (R) form organisms devoid of O antigen by all usually applied criteria, the antigen contained a small amount of smooth form LPS, most probably derived from a slight leakiness of the rfa mutation responsible for the R character. The small amount of contaminating O antigen was apparently rendered highly immunogenic by complexation with the outer membrane protein.