| Literature DB >> 24698274 |
Michael Ailion1, Mandy Hannemann2, Susan Dalton3, Andrea Pappas3, Shigeki Watanabe3, Jan Hegermann4, Qiang Liu3, Hsiao-Fen Han3, Mingyu Gu3, Morgan Q Goulding5, Nikhil Sasidharan6, Kim Schuske3, Patrick Hullett3, Stefan Eimer4, Erik M Jorgensen7.
Abstract
Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network.Entities:
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Year: 2014 PMID： 24698274 PMCID： PMC3997996 DOI： 10.1016/j.neuron.2014.02.017
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173