OBJECTIVES: The aim of this study was to determine the agreement between AB blood phenotyping and genotyping and determine whether non-AB blood type incompatibilities exist in UK cats. METHODS: Blood samples underwent phenotyping (A, B or AB) using microplate agglutination, and genotyping (AA, Ab or bb) using pyrosequencing of a fragment of the cytidine monophospho-N-acetylneuraminic acid hydroxylase gene. Non-AB blood type incompatibilities were investigated by cross-matching against reference blood of the same phenotype. RESULTS: Of 112 cats tested, 86 (77%) were blood phenotype A, 19 (17%) type B and 7 (6%) type AB. Genotype and initial phenotype agreed in 96% (107 of 112) of cats, but 5 were discordant; these were all B phenotype with either AA (n=2) or Ab (n=3) genotype. Two of the five cats had repeat blood samples tested: one was reclassified as phenotype A; the other remained phenotype B. Two cats had incompatibilities on minor cross-match, but these were attributed to phenotyping errors. CLINICAL SIGNIFICANCE: Unknown mutation(s) associated with phenotype B, resulting in false AA or Ab genotyping, were evident in a small number of cases in this study. No conclusive evidence for non-AB blood type incompatibilities was found.
OBJECTIVES: The aim of this study was to determine the agreement between AB blood phenotyping and genotyping and determine whether non-AB blood type incompatibilities exist in UK cats. METHODS: Blood samples underwent phenotyping (A, B or AB) using microplate agglutination, and genotyping (AA, Ab or bb) using pyrosequencing of a fragment of the cytidine monophospho-N-acetylneuraminic acid hydroxylase gene. Non-AB blood type incompatibilities were investigated by cross-matching against reference blood of the same phenotype. RESULTS: Of 112 cats tested, 86 (77%) were blood phenotype A, 19 (17%) type B and 7 (6%) type AB. Genotype and initial phenotype agreed in 96% (107 of 112) of cats, but 5 were discordant; these were all B phenotype with either AA (n=2) or Ab (n=3) genotype. Two of the five cats had repeat blood samples tested: one was reclassified as phenotype A; the other remained phenotype B. Two cats had incompatibilities on minor cross-match, but these were attributed to phenotyping errors. CLINICAL SIGNIFICANCE: Unknown mutation(s) associated with phenotype B, resulting in false AA or Ab genotyping, were evident in a small number of cases in this study. No conclusive evidence for non-AB blood type incompatibilities was found.
Authors: Heidi Anderson; Stephen Davison; Katherine M Lytle; Leena Honkanen; Jamie Freyer; Julia Mathlin; Kaisa Kyöstilä; Laura Inman; Annette Louviere; Rebecca Chodroff Foran; Oliver P Forman; Hannes Lohi; Jonas Donner Journal: PLoS Genet Date: 2022-06-16 Impact factor: 6.020
Authors: Barbara Gandolfi; Robert A Grahn; Nicholas A Gustafson; Daniela Proverbio; Eva Spada; Badri Adhikari; Janling Cheng; Gordon Andrews; Leslie A Lyons; Chris R Helps Journal: PLoS One Date: 2016-05-12 Impact factor: 3.240
Authors: Isabelle Goy-Thollot; Alexandra Nectoux; Maryline Guidetti; Benjamin Chaprier; Sarah Bourgeois; Catherine Boisvineau; Anthony Barthélemy; Céline Pouzot-Nevoret; Urs Giger Journal: J Vet Intern Med Date: 2018-12-17 Impact factor: 3.333
Authors: Hüseyin Can; Sedef Erkunt Alak; Ahmet Efe Köseoğlu; Umut Şahar; Berna Bostanbaş; Serdar Baydarlı; Mert Döşkaya; Cemal Ün Journal: BMC Genomics Date: 2021-04-19 Impact factor: 3.969
Authors: Megan E McClosky; Dorothy Cimino Brown; Nicole M Weinstein; Nicole Chappini; Michael T Taney; Kimberly Marryott; Mary Beth Callan Journal: J Vet Intern Med Date: 2018-10-11 Impact factor: 3.333
Authors: Alexandra Nectoux; Maryline Guidetti; Anthony Barthélemy; Céline Pouzot-Nevoret; Guillaume L Hoareau; Isabelle Goy-Thollot Journal: JFMS Open Rep Date: 2019-07-22