| Literature DB >> 24696349 |
Emily Plummer1, Sujan Shresta.
Abstract
Validation of a mouse model of dengue virus (DENV) infection relies on verification of viremia and productive replication in mouse tissues following infection. Here, we describe a quantitative assay for determining viral RNA levels in mouse serum and tissues. For the purpose of confirming DENV replication, we outline a fluorescence immunohistochemistry (FIHC) protocol for staining a nonstructural protein of DENV.Entities:
Mesh：
Substances：
Year: 2014 PMID： 24696349 DOI： 10.1007/978-1-4939-0348-1_23
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745