Literature DB >> 2469625

Molecular cloning of mouse protein kinase C (PKC) cDNA from Swiss 3T3 fibroblasts.

S Rose-John1, A Dietrich, F Marks.   

Abstract

A set of complementary DNAs (cDNAs) that encode the complete 672-amino acid sequence for mouse protein kinase C (PKC) type alpha have been isolated from a mouse Swiss 3T3 cDNA library. Extensive rescreening of this cDNA library only resulted in the isolation of clones of the same PKC species, indicating that Swiss 3T3 fibroblasts express exclusively PKC-alpha. This enzyme is encoded by two different mRNAs that exhibit a substantial heterogeneity in their 3'-noncoding regions. This heterogeneity could have been derived from alternative splicing of the pre-mRNAs or be due to differential usage of the polyadenylation motif. The expression of PKC mRNA in fibroblasts is very low and it is not influenced by treatment with the tumor promoter 12-O-tetradecanoyl-13-phorbol-acetate.

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Year:  1988        PMID： 2469625     DOI： 10.1016/0378-1119(88)90179-5

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  32 in total

1.  Mechanism of interleukin-1- and tumor necrosis factor alpha-dependent regulation of the alpha 1-antichymotrypsin gene in human astrocytes.

Authors:  T Kordula; M Bugno; R E Rydel; J Travis
Journal:  J Neurosci       Date:  2000-10-15       Impact factor: 6.167

2.  Characterization of the spermidine-dependent, sequence-specific endoribonuclease that requires transfer RNA for its activity.

Authors:  M Nashimoto
Journal:  Nucleic Acids Res       Date:  1992-07-25       Impact factor: 16.971

3.  Isolation of cloned mouse protein kinase C beta-II cDNA and its sequence.

Authors:  Y M Tang; C L Ashendel
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

Review 4.  An analysis of vertebrate mRNA sequences: intimations of translational control.

Authors:  M Kozak
Journal:  J Cell Biol       Date:  1991-11       Impact factor: 10.539

5.  Simultaneous visualization of the translocation of protein kinase Calpha-green fluorescent protein hybrids and intracellular calcium concentrations.

Authors:  K Almholt; P O Arkhammar; O Thastrup; S Tullin
Journal:  Biochem J       Date:  1999-01-15       Impact factor: 3.857

6.  Protein kinase C isozymes epsilon and alpha in murine erythroleukemia cells.

Authors:  C T Powell; L Leng; L Dong; H Kiyokawa; X Busquets; K O'Driscoll; P A Marks; R A Rifkind
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

7.  Protein kinase C isoenzymes in airway smooth muscle.

Authors:  B L Webb; M A Lindsay; P J Barnes; M A Giembycz
Journal:  Biochem J       Date:  1997-05-15       Impact factor: 3.857

8.  Sequence of human protein kinase C alpha.

Authors:  G Finkenzeller; D Marmé; H Hug
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

9.  Molecular cloning and expression of an intracellular serpin: an elastase inhibitor from horse leucocytes.

Authors:  T Kordula; A Dubin; H Schooltink; A Koj; P C Heinrich; S Rose-John
Journal:  Biochem J       Date:  1993-07-01       Impact factor: 3.857

10.  Inhibition of protein kinase C-alpha expression in mice after systemic administration of phosphorothioate antisense oligodeoxynucleotides.

Authors:  N M Dean; R McKay
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-22       Impact factor: 11.205
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