Regulation of cGMP-dependent conductance in cytoplasmic membrane of rod outer segments by transducin.

A preparation of the photoreceptor G-protein, transducin, containing mainly the T alpha-subunit in a GTP-gamma-S-bound form, has been used for perfusion of the intracellular surface of excised patches of rod outer segment cytoplasmic membrane from frog retina. The preparation has been shown to result in the complete suppression of the cGMP-activated ionic conductance of the cytoplasmic membrane patch. The effect is entirely reversible after the protein has been washed out and is not observed in the absence of cGMP. The degree of conductance inhibition depends on the protein concentration, half-maximal inhibition occurring at 1 microM T alpha-GTP-gamma-S.