| Literature DB >> 24692496 |
Abstract
This protocol uses T4 polynucleotide kinase to catalyze the transfer of a radiolabeled, terminal (γ) phosphate of ATP to the 5'-hydroxyl terminus of a DNA or RNA molecule. The reaction is very efficient and hence is used as a general method for phosphorylating polynucleotides or oligonucleotides.Entities:
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Year: 2014 PMID： 24692496 DOI： 10.1101/pdb.prot080739
Source DB: PubMed Journal: Cold Spring Harb Protoc ISSN： 1559-6095