Literature DB >> 24690559

A simple and effective coumarin-based fluorescent probe for cysteine.

Xi Dai1, Qing-Hua Wu2, Peng-Chong Wang2, Jie Tian1, Yu Xu1, Sheng-Qing Wang1, Jun-Ying Miao3, Bao-Xiang Zhao4.   

Abstract

Acrylic acid 3-acetyl-2-oxo-2 H-chromen-7-yl ester (ACA) was rationally designed and synthesized as a simple and effective fluorescent probe for sensing cysteine with high selectivity and naked-eye detection. The probe can detect cysteine by fluorescence spectrometry with a detection limit of 0.657 μM and can be used with calf serum and in live cell imaging. The conjugate addition/cyclization sequence mechanism of the reaction between ACA and cysteine was confirmed by ESI-MS and fluorescence spectra.
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Calf serum; Cell imaging; Conjugate addition; Coumarin; Cysteine; Fluorescent probe

Mesh：

Substances：

Year:  2014        PMID： 24690559     DOI： 10.1016/j.bios.2014.03.018

Source DB:  PubMed          Journal:  Biosens Bioelectron        ISSN： 0956-5663            Impact factor:   10.618


  9 in total

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  9 in total

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