Literature DB >> 24688178

Evaluation of a real-time polymerase chain reaction assay of the outer membrane protein P2 gene for the detection of Haemophilus parasuis in clinical samples.

Rebeccah McDowall1, Durda Slavic1, Janet I MacInnes1, Hugh Y Cai1.   

Abstract

A real-time polymerase chain reaction (PCR) assay of the outer membrane protein (OMP) P2 gene was developed and used to test 97 putative Haemophilus parasuis pure cultures and 175 clinical tissue samples. With standard culture isolation as the gold standard, the diagnostic sensitivity and specificity of the PCR assay were determined to be 83% and 80%, respectively.

Entities:  

Mesh：

Substances：

Year:  2014        PMID： 24688178      PMCID： PMC3962279     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  8 in total

1.  16S ribosomal RNA sequence-based identification of veterinary clinical bacteria.

Authors:  Hugh Cai; Marie Archambault; John F Prescott
Journal:  J Vet Diagn Invest       Date:  2003-09       Impact factor: 1.279

2.  Molecular cloning, sequencing, and expression of the outer membrane protein P2 gene of Haemophilus parasuis.

Authors:  Peng Li; Juan Bai; Jun-xing Li; Guo-long Zhang; Yan-hua Song; Yu-feng Li; Xian-wei Wang; Ping Jiang
Journal:  Res Vet Sci       Date:  2011-09-25       Impact factor: 2.534

3.  Development of an improved species specific PCR test for detection of Haemophilus parasuis.

Authors:  Oystein Angen; Simone Oliveira; Peter Ahrens; Birgitta Svensmark; Thomas D Leser
Journal:  Vet Microbiol       Date:  2006-11-20       Impact factor: 3.293

4.  Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heat-stable antigen extracts.

Authors:  P Kielstein; V J Rapp-Gabrielson
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

5.  Validation of a real-time PCR for Haemophilus parasuis.

Authors:  C Turni; M Pyke; P J Blackall
Journal:  J Appl Microbiol       Date:  2009-08-19       Impact factor: 3.772

6.  Development of a PCR test to diagnose Haemophilus parasuis infections.

Authors:  S Oliveira; L Galina; C Pijoan
Journal:  J Vet Diagn Invest       Date:  2001-11       Impact factor: 1.279

7.  Comparison of sampling sites and detection methods for Haemophilus parasuis.

Authors:  C Turni; P J Blackall
Journal:  Aust Vet J       Date:  2007-05       Impact factor: 1.281

8.  Prevalence of Actinobacillus pleuropneumoniae, Actinobacillus suis, Haemophilus parasuis, Pasteurella multocida, and Streptococcus suis in representative Ontario swine herds.

Authors:  Janet I MacInnes; Marcelo Gottschalk; Abdul G Lone; Devon S Metcalf; Shivani Ojha; Thomas Rosendal; Sheila B Watson; Robert M Friendship
Journal:  Can J Vet Res       Date:  2008-04       Impact factor: 1.310
  8 in total
  2 in total

1.  Comparison of Pneumocystis nucleic acid and antibody profiles and their associations with other respiratory pathogens in two Austrian pig herds.

Authors:  Christiane Weissenbacher-Lang; Nora Nedorost; Christian Knecht; Isabel Hennig-Pauka; Mathias Huber; Thomas Voglmayr; Herbert Weissenböck
Journal:  PLoS One       Date:  2017-09-25       Impact factor: 3.240

2.  Development of a recombinase polymerase amplification assay for rapid detection of Haemophilus parasuis in tissue samples.

Authors:  Qiaoyi Han; Jinfeng Wang; Ruiwen Li; Qingan Han; Wanzhe Yuan; Jianchang Wang
Journal:  Vet Med Sci       Date:  2020-05-26
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.