Vinod Agrawal1, Ms Rama Rao2, Kanupriya Dhingra3, V Rajesh Gopal4, Abhijita Mohapatra5, Abhilash Mohapatra6. 1. Professor and Head, Department of Conservative Dentistry and Endodontics, Triveni Institute of Dental Sciences and Research Centre Bilaspur, Chhattisgarh, India. 2. Professor and Head, Department of Conservative Dentistry and Endodontics, Sree Sai Dental College and Research Institute Srikakulam, Andhra Pradesh, India. 3. Consultant Endodontist, Mumbai, Maharashtra, India, e-mail: kp.dhingra@gmail.com. 4. Professor and Head, Department of Conservative Dentistry and Endodontics, Best Dental Science College, Melur Road, Ultra Nagar Madurai, Tamil Nadu, India. 5. Reader, Department of Prosthodontics, Institute of Dental Sciences Khandagiri, Bhubaneswar, Odisha, India. 6. Senior Lecturer, Department of Prosthodontics, Institute of Dental Sciences, Khandagiri, Bhubaneswar, Odisha, India.
Abstract
AIM: This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a fnal rinse against Enterococcus faecalis. MATERIALS AND METHODS: Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours. RESULTS: Statistical analysis showed that there was a signifcant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation. CONCLUSION: The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX. CLINICAL SIGNIFCANCE: E. faecalis is one of the most resistant intracanal species and a possible cause of root canal failure. Many authors have stressed the importance of using antimicrobial irrigants during chemomechanical preparation to ensure complete disinfection. Therefore, various irrigating solutions have been used during and immediately after root canal preparation to remove debris and necrotic pulp tissue and to eliminate microorganisms that cannot be reached by mechanical instrumentation.
AIM: This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a fnal rinse against Enterococcus faecalis. MATERIALS AND METHODS: Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours. RESULTS: Statistical analysis showed that there was a signifcant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation. CONCLUSION: The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX. CLINICAL SIGNIFCANCE: E. faecalis is one of the most resistant intracanal species and a possible cause of root canal failure. Many authors have stressed the importance of using antimicrobial irrigants during chemomechanical preparation to ensure complete disinfection. Therefore, various irrigating solutions have been used during and immediately after root canal preparation to remove debris and necrotic pulp tissue and to eliminate microorganisms that cannot be reached by mechanical instrumentation.