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Literature DB >> 24685601

Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing.

Olivier Clermont¹, Julia K Christenson², Anne-Sophie Daubié³, David M Gordon², Erick Denamur⁴.

Abstract

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

Entities: Species

Keywords: Allele-specific PCR; B2 phylogroup; B2 sub-group; Escherichia coli; Sequence type complex

Mesh：

Substances：
DNA, Bacterial

Year: 2014 PMID： 24685601 DOI： 10.1016/j.mimet.2014.03.008

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

32 in total

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Journal: Antimicrob Agents Chemother Date: 2020-09-21 Impact factor: 5.191

2. The Pandemic H30 Subclone of Sequence Type 131 (ST131) as the Leading Cause of Multidrug-Resistant Escherichia coli Infections in the United States (2011-2012).

Authors: James R Johnson; Stephen Porter; Paul Thuras; Mariana Castanheira
Journal: Open Forum Infect Dis Date: 2017-05-02 Impact factor: 3.835

3. The rpoS gene is predominantly inactivated during laboratory storage and undergoes source-sink evolution in Escherichia coli species.

Authors: Alexandre Bleibtreu; Olivier Clermont; Pierre Darlu; Jérémy Glodt; Catherine Branger; Bertrand Picard; Erick Denamur
Journal: J Bacteriol Date: 2014-09-29 Impact factor: 3.490

4. Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli, Including Sequence Type 131 (ST131), from Retail Chicken Breasts in the United States in 2013.

Authors: James R Johnson; Stephen B Porter; Brian Johnston; Paul Thuras; Sarah Clock; Michael Crupain; Urvashi Rangan
Journal: Appl Environ Microbiol Date: 2017-03-02 Impact factor: 4.792

5. Escherichia coli B2 Phylogenetic Subgroups in the Infant Gut Microbiota: Predominance of Uropathogenic Lineages in Swedish Infants and Enteropathogenic Lineages in Pakistani Infants.

Authors: Forough L Nowrouzian; Olivier Clermont; Mona Edin; Anna Östblom; Erick Denamur; Agnes E Wold; Ingegerd Adlerberth
Journal: Appl Environ Microbiol Date: 2019-11-27 Impact factor: 4.792

6. Extensive Household Outbreak of Urinary Tract Infection and Intestinal Colonization due to Extended-Spectrum β-Lactamase-Producing Escherichia coli Sequence Type 131.

Authors: Theresa Madigan; James R Johnson; Connie Clabots; Brian D Johnston; Stephen B Porter; Billie S Slater; Ritu Banerjee
Journal: Clin Infect Dis Date: 2015-03-31 Impact factor: 9.079

7. Epidemic Emergence in the United States of Escherichia coli Sequence Type 131-H30 (ST131-H30), 2000 to 2009.

Authors: James R Johnson; Stephen Porter; Paul Thuras; Mariana Castanheira
Journal: Antimicrob Agents Chemother Date: 2017-07-25 Impact factor: 5.191

8. Variation in resistance traits, phylogenetic backgrounds, and virulence genotypes among Escherichia coli clinical isolates from adjacent hospital campuses serving distinct patient populations.

Authors: Sarah M Drawz; Stephen Porter; Michael A Kuskowski; Brian Johnston; Connie Clabots; Susan Kline; Patricia Ferrieri; James R Johnson
Journal: Antimicrob Agents Chemother Date: 2015-06-22 Impact factor: 5.191

9. Isolation and Characterization of Escherichia coli Sequence Type 131 and Other Antimicrobial-Resistant Gram-Negative Bacilli from Clinical Stool Samples from Veterans.

Authors: Muhanad Mohamed; Connie Clabots; Stephen B Porter; Paul Thuras; James R Johnson
Journal: Antimicrob Agents Chemother Date: 2016-07-22 Impact factor: 5.191

10. Activity of Imipenem-Relebactam against Carbapenem-Resistant Escherichia coli Isolates from the United States in Relation to Clonal Background, Resistance Genes, Coresistance, and Region.